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• W3149443203 abstract "Abstract Maintaining the integrity of the cell plasma membrane (PM) is critical for the survival of cells. While an efficient PM repair machinery can aid survival of healthy cells by preventing influx of extracellular calcium, it can also constitute an obstacle in drug delivery and photothermal therapy. We show how nanoscopic holes can be applied to the cell surface thus allowing identification of molecular components with a pivotal role in PM repair. Cells are punctured by locally heating gold nanostructures at the cell surface which causes nano-ruptures in cellular PMs. Recruitment of annexin V near the hole is found to locally reshape the ruptured plasma membrane. Experiments using model membranes, containing recombinant annexin V, provide further biophysical insight into the ability of annexin V to reshape edges surrounding a membrane hole. The thermoplasmonic method provides a general strategy to monitor the response to nanoscopic injuries to the cell surface." @default.
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• W3149443203 date "2021-03-28" @default.
• W3149443203 modified "2023-10-14" @default.
• W3149443203 title "Thermoplasmonic nano-rupture of cells reveals Annexin V function in plasma membrane repair" @default.
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• W3149443203 doi "https://doi.org/10.1101/2021.03.26.436963" @default.
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