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- W3149655096 abstract "Background: EGFR mutant adenocarcinoma accounts for almost 33% cases of NSCLC in the Asian population. Single gene testing on tissue blocks has been the gold standard since its discovery, however owing to the inherent technical difficulties, invasive nature, difficult accessibility to the lung may limit its use in many patients. Liquid biopsy has evolved as a value added tool for early diagnosis of biomarkers in lung cancer. Various detection methods have been employed for the detection of EGFR mutation like Cobas V2 and ddPCR. This study was done to evaluate the concordance of these techniques against the tissue biopsy for primary EGFR mutation detection. Methods: 117 patients of NSCLC between April 2020-September 2020 were evaluated for primary EGFR mutations by therascreen, DDPCR and Cobas V2. Statistical analysis was done using SPSS version 23, using descriptive statistics and kappa agreement. Results: Primary EGFR testing was done in 117 samples. The positivity rate for cobas was 45.6% and that for ddpCR was 28.6%. The concordance between tissue and cobas EGFR mutation was 96.33%. Considering tissue as gold standard, the ROC analysis depicted an AUC of 0.923 for cobas and 0.800 for DDPCR, implying a sensitivity of ~92% for cobas and 80% for DDPCR. Mutation specific sensitivities also showed similar trends, except for T790M for which sensitivity of ddPCR was 50% with a specificity of 100% compared to 50% and 80% with cobas. Uncommon mutations had 0% sensitivity and specificity for ddPCR, as these cannot be detected by this assay. The AUC for ddpcr vs cobas was 0.754. All the p values were <0.001. Two cases were negative by all three assays and were positive on NGS as these were non-canonical and nonhotspot mutations not incorporated in the primer sets of any of the assays in question. The Z statistics score was 25.4 and Cronbachs alpha was 0.973 for cobas compared to tissue, and they were 6.91 and 0.8008 for ddPCR respectively. Conclusions: From this first experience from India, the advantages of upfront liquid biopsy are evident, and adopting the same, may prove promising in a clinician's armamentarium. Cobas has advantages of broader mutation spectrum compared to ddPCR, however is limited by sensitivity, whereas ddPCR is limited by specificity although can provide absolute quantification. Legal entity responsible for the study: Ullas Batra. Funding: Has not received any funding. Disclosure: All authors have declared no conflicts of interest." @default.
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- W3149655096 date "2021-04-01" @default.
- W3149655096 modified "2023-10-16" @default.
- W3149655096 title "39P Tissue vs. cobas vs. DDPCR for EGFR in NSCLC: Real-world evidence from India" @default.
- W3149655096 doi "https://doi.org/10.1016/s1556-0864(21)01881-5" @default.
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