Matches in SemOpenAlex for { <https://semopenalex.org/work/W3149747160> ?p ?o ?g. }
- W3149747160 abstract "ABSTRACT Dopaminergic neurons are critical to movement, mood, addiction, and stress. Current techniques for generating dopaminergic neurons from human induced pluripotent stem cells (hiPSCs) yield heterogenous cell populations with variable purity and inconsistent reproducibility between donors, hiPSC clones, and experiments. Here, we report the rapid (5 weeks) and efficient (~90%) induction of induced dopaminergic neurons (iDANs) through transient overexpression of lineage-promoting transcription factors combined with stringent selection across five donors. We observe maturation-dependent increase in dopamine synthesis, together with electrophysiological properties consistent with midbrain dopaminergic neuron identity, such as slow-rising after hyperpolarization potentials, an action potential duration of ~3ms, tonic sub-threshold oscillatory activity, and spontaneous burst firing at frequency of ~1.0-1.75 Hz. Transcriptome analysis reveals robust expression of genes involved in fetal midbrain dopaminergic neuron identity. Specifically expressed genes in iDANs, relative to their isogenic glutamatergic and GABAergic counterparts, were linked to the genetic risk architecture of a broad range of psychiatric traits, with iDANs showing particularly strong enrichment in loci conferring heritability for cannabis use disorder, schizophrenia, and bipolar disorder. Therefore, iDANs provide a critical tool for modeling midbrain dopaminergic neuron development and dysfunction in psychiatric disease." @default.
- W3149747160 created "2021-04-13" @default.
- W3149747160 creator A5005687826 @default.
- W3149747160 creator A5006025541 @default.
- W3149747160 creator A5008237860 @default.
- W3149747160 creator A5015044919 @default.
- W3149747160 creator A5015295333 @default.
- W3149747160 creator A5025600687 @default.
- W3149747160 creator A5040099157 @default.
- W3149747160 creator A5047494259 @default.
- W3149747160 creator A5048087515 @default.
- W3149747160 creator A5059987827 @default.
- W3149747160 creator A5070372173 @default.
- W3149747160 creator A5074392365 @default.
- W3149747160 creator A5083220171 @default.
- W3149747160 creator A5085780704 @default.
- W3149747160 creator A5085972715 @default.
- W3149747160 date "2021-04-02" @default.
- W3149747160 modified "2023-09-26" @default.
- W3149747160 title "Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease Risk" @default.
- W3149747160 cites W1917316786 @default.
- W3149747160 cites W1976088739 @default.
- W3149747160 cites W1986058969 @default.
- W3149747160 cites W1987167495 @default.
- W3149747160 cites W2014444260 @default.
- W3149747160 cites W2040027262 @default.
- W3149747160 cites W2045227722 @default.
- W3149747160 cites W2051314902 @default.
- W3149747160 cites W2072372271 @default.
- W3149747160 cites W2081098333 @default.
- W3149747160 cites W2083796374 @default.
- W3149747160 cites W2085957678 @default.
- W3149747160 cites W2089993812 @default.
- W3149747160 cites W2096193724 @default.
- W3149747160 cites W2100761975 @default.
- W3149747160 cites W2103017472 @default.
- W3149747160 cites W2105973849 @default.
- W3149747160 cites W2107018762 @default.
- W3149747160 cites W2112220625 @default.
- W3149747160 cites W2114104545 @default.
- W3149747160 cites W2114206069 @default.
- W3149747160 cites W2114570899 @default.
- W3149747160 cites W2130116522 @default.
- W3149747160 cites W2130128490 @default.
- W3149747160 cites W2137526110 @default.
- W3149747160 cites W2138207763 @default.
- W3149747160 cites W2142410410 @default.
- W3149747160 cites W2143541037 @default.
- W3149747160 cites W2146512944 @default.
- W3149747160 cites W2156640524 @default.
- W3149747160 cites W2169456326 @default.
- W3149747160 cites W2178051376 @default.
- W3149747160 cites W2293324340 @default.
- W3149747160 cites W2336873653 @default.
- W3149747160 cites W2340656051 @default.
- W3149747160 cites W2406427124 @default.
- W3149747160 cites W2528543174 @default.
- W3149747160 cites W2536580992 @default.
- W3149747160 cites W2559588208 @default.
- W3149747160 cites W2586273122 @default.
- W3149747160 cites W2613022045 @default.
- W3149747160 cites W2613062475 @default.
- W3149747160 cites W2618849273 @default.
- W3149747160 cites W2717351480 @default.
- W3149747160 cites W2730417813 @default.
- W3149747160 cites W2739834351 @default.
- W3149747160 cites W2745116209 @default.
- W3149747160 cites W2768166779 @default.
- W3149747160 cites W2773591768 @default.
- W3149747160 cites W2774477442 @default.
- W3149747160 cites W2787933178 @default.
- W3149747160 cites W2791854743 @default.
- W3149747160 cites W2792717260 @default.
- W3149747160 cites W2796465642 @default.
- W3149747160 cites W2802815532 @default.
- W3149747160 cites W2803649084 @default.
- W3149747160 cites W2901489210 @default.
- W3149747160 cites W2911487850 @default.
- W3149747160 cites W2914482125 @default.
- W3149747160 cites W2950375827 @default.
- W3149747160 cites W2950464315 @default.
- W3149747160 cites W2952673268 @default.
- W3149747160 cites W2953357410 @default.
- W3149747160 cites W2979994192 @default.
- W3149747160 cites W2986932567 @default.
- W3149747160 cites W2991410918 @default.
- W3149747160 cites W2995971356 @default.
- W3149747160 cites W3002402252 @default.
- W3149747160 cites W3006409861 @default.
- W3149747160 cites W3008757859 @default.
- W3149747160 cites W3012542319 @default.
- W3149747160 cites W3036739968 @default.
- W3149747160 cites W3037544130 @default.
- W3149747160 cites W3080632072 @default.
- W3149747160 cites W3085447556 @default.
- W3149747160 cites W3087062906 @default.
- W3149747160 cites W3092754274 @default.
- W3149747160 cites W3094181094 @default.
- W3149747160 cites W3094864623 @default.
- W3149747160 cites W3112600195 @default.