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- W3149767551 abstract "The electrophoretic mobility shift assay is a useful tool to identify proteins and nucleic acids interac- tions. Traditionally, the nucleic acids fragments are end- labeled with 32 P. We present here the use of fluorescent methodologies for studies of RNA in place of radioactivity. The method is highly sensitive and quantitative with the use of an infrared fluorescence imaging system. Fluores- cently labeled primers can be used to monitor protein- RNA interactions by fluorescent mobility shift assays. The simplicity and validity of this approach may have more advantages than that of previous methods that traditionally used hazardous radioisotopes. This method was success- fully tested in the study of the interactions between MS2 Coat Protein and its RNA target." @default.
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- W3149767551 date "2010-01-01" @default.
- W3149767551 modified "2023-09-27" @default.
- W3149767551 title "Using FAM labeled DNA oligos to do RNA electrophoretic mobility shift assay" @default.
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