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• W3149784768 abstract "An expressed sequence tag (EST) highly similar to BdPT1-4, a phosphate transporter (PT) gene in Brachypodium distachyon, was obtained in a wheat root cDNA subtractive suppression library containing genes that respondtolow-phosphate(Pi)stress.TheDNAsequencecoveringthisEST(designatedasTaPht1;4)wasdeterminedbased on screening a wheat DNA library. TaPht1;4 consists of two exons and one intron and encodes a 555 amino acid (aa) polypeptide with a molecular weight of 60.85kDa and an isoelectric point of 7.60. TaPht1;4 contains 12 conserved membrane-spanningdomainssimilartopreviouslyreportedPTsindiverseplantspecies.Yeastcomplementanalysisinlow- Pi mediumconfirmed thatTaPht1;4confersthe capacitytouptakePi toMB192,a yeaststrain withadefective high-affinity PT;withanapparentKmof35.3 mM.TheTaPht1;4transcriptswerespecificallydetectedintherootandwerehighlyinduced under Pi deficiency.TaPht1;4was alsoexpressed followinga diurnalpattern,i.e.highlevels duringdaytime andlowlevels during night-time. TaPht1;4 overexpression and downregulation dramatically altered the plant phenotypic features under low-Piconditions.SamplesthatoverexpressedTaPht1;4hadsignificantlyimprovedgrowthtraitsandaccumulatedmorePi thanthewild-type plantandthose withdownregulatedTaPht1;4expression.Therefore,TaPht1;4isahigh-affinityPTgene that plays a critical role in wheat Pi acquisition under Pi deprivation." @default.
• W3149784768 created "2021-04-13" @default.
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• W3149784768 date "2013-01-01" @default.
• W3149784768 modified "2023-10-02" @default.
• W3149784768 title "TaPht1;4, a high-affinity phosphate transporter gene in wheat (Triticum aestivum), plays an important role in plant phosphate acquisition under phosphorus deprivation" @default.
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