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- W3149795271 abstract "The 3'-truncated preS/S region from HBV genome, encoding a trans criptional transactivator, was cloned and a recombination expression vector for 3'- truncated HBV preS/S sequences under the control of the CMV promoter was constructed by recombination DNA techniques. Then, the expression vector DNA was microinjected into pronuclei of fertilized mouse oocytes. Founders of transgenic mice harbouring the recombination gene which can be expressed were screened by polymerase chain reaction (PCR) at genomic DNA level and confirmed by ELISA andlysis at protein level. Two of 15 mice in one series of microinjections showed the expression of 3'-truncated preS/S gene from HBV genome. The expression vector of 3' -truncated preS/S gene might be helpful for further studies of relationship between the expression preduction of 3'-truncared preS/ S sequence and HBV-associated onco- genesis in vitro. The transgenic mice with expressing 3'-truncated preS/S gene will provide new insight into its roles in the development of human hepatocelluar carcinoma (HCC) in vivo." @default.
- W3149795271 created "2021-04-13" @default.
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- W3149795271 date "2000-01-01" @default.
- W3149795271 modified "2023-09-23" @default.
- W3149795271 title "Expression of 3'-truncated preS/ S Gene in Transgenic Mice" @default.
- W3149795271 hasPublicationYear "2000" @default.
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