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- W3149804521 abstract "EKLF 是一 erythroid 特定，锌为在在权威的系的 erythroid 房间的基因的哺乳动物的贝它滴的激活的包含手指的抄写因素必需品。我们准备了对西方的弄污和免疫降水(IP ) 质量合适的 polyclonal 反老鼠 EKLF 抗体，并且使用了它在老鼠 erythroid 开发期间定义 EKLF 蛋白质的表示模式。我们也为 chromatin-immunoprecipitation (薄片) 使用了这抗体试金。EKLF 被发现以一种 DMSO 可诱导的方式在老鼠 erythroleukemia 房间(MEL ) 在老鼠 beta-major-globin 倡导者和 beta-LCR 的 HS2 地点在 vivo 绑。象三另外的蛋白质一样的 EKLF 的 导致DMSO 的绑定，也就是 RNA 聚合酶 II ，乙酰 ated 嘘一 H3 ，和 methylated 嘘一 H3 ，没在 CB3 被废除，但是显著地降低了，一根 导出MEL 的房间线与 p45/NF-E2 空表示，在 loci 为哺乳动物的滴的激活需要的一个 充实erythroid 的因素。有趣地，在 vivo 的 EKLF 的绑定也在地点在鼠标象 alpha 一样滴被检测，在成年高山哈在倡导者和它的远在上游的规章的元素 alpha-MRE (HS26 ) 的滴。这研究提供直接证据为在在在基因的像贝它的滴聚类的老鼠的主要规章的元素的 vivo EKLF 有约束力数据也在基因规定在哺乳动物的滴关于 EKLF 染色质相互作用的角色有有趣的含意。" @default.
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- W3149804521 date "2006-01-01" @default.
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- W3149804521 title "Chromatin-binding in vivo of the erythroid kruppel-like factor, EKLF, in the murine globin loci" @default.
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