Matches in SemOpenAlex for { <https://semopenalex.org/work/W3149933165> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3149933165 endingPage "210" @default.
- W3149933165 startingPage "207" @default.
- W3149933165 abstract "Efficient gene transfer by cytoplasm co-injec-tion will offer a powerful means for transgenic animals. Us-ing co-injection in cytoplasm, two independent gene con-structs, including bovine a-s1-casein-hG-CSF and a mammal expression vector expressing a nuclear localization signal (mNLS), were introduced into fertilized mouse eggs. The target gene construct was docked into host nucleus probably by the nuclear localization signal. Transgene mice have been obtained at 58% (29/50) of integration ratio. Ex-pression level of the positive transgene mice was detected by Western blotting. Maximal expression of human G-CSF was estimated about 540 mg/L of milk. The expression ratio was up to 75% (9/12). The results here have important practical implications for the generation of mammary gland bioreac-tors and other transgene studies. Co-injection of a target gene with an expression vector of a mammal nuclear localization signal by cytoplasm appears to be a useful, efficient and easy strategy for generating transgenic" @default.
- W3149933165 created "2021-04-13" @default.
- W3149933165 creator A5001978013 @default.
- W3149933165 creator A5005035346 @default.
- W3149933165 creator A5022487124 @default.
- W3149933165 creator A5027921459 @default.
- W3149933165 creator A5032245741 @default.
- W3149933165 creator A5053780153 @default.
- W3149933165 creator A5064289650 @default.
- W3149933165 creator A5066455265 @default.
- W3149933165 creator A5069719675 @default.
- W3149933165 creator A5073161777 @default.
- W3149933165 creator A5085346496 @default.
- W3149933165 creator A5085354021 @default.
- W3149933165 date "2002-01-01" @default.
- W3149933165 modified "2023-09-23" @default.
- W3149933165 title "Application of a nuclear localization signal gene in transgene mice" @default.
- W3149933165 hasPublicationYear "2002" @default.
- W3149933165 type Work @default.
- W3149933165 sameAs 3149933165 @default.
- W3149933165 citedByCount "0" @default.
- W3149933165 crossrefType "journal-article" @default.
- W3149933165 hasAuthorship W3149933165A5001978013 @default.
- W3149933165 hasAuthorship W3149933165A5005035346 @default.
- W3149933165 hasAuthorship W3149933165A5022487124 @default.
- W3149933165 hasAuthorship W3149933165A5027921459 @default.
- W3149933165 hasAuthorship W3149933165A5032245741 @default.
- W3149933165 hasAuthorship W3149933165A5053780153 @default.
- W3149933165 hasAuthorship W3149933165A5064289650 @default.
- W3149933165 hasAuthorship W3149933165A5066455265 @default.
- W3149933165 hasAuthorship W3149933165A5069719675 @default.
- W3149933165 hasAuthorship W3149933165A5073161777 @default.
- W3149933165 hasAuthorship W3149933165A5085346496 @default.
- W3149933165 hasAuthorship W3149933165A5085354021 @default.
- W3149933165 hasConcept C102230213 @default.
- W3149933165 hasConcept C104317684 @default.
- W3149933165 hasConcept C141035611 @default.
- W3149933165 hasConcept C150194340 @default.
- W3149933165 hasConcept C153911025 @default.
- W3149933165 hasConcept C190062978 @default.
- W3149933165 hasConcept C2776879804 @default.
- W3149933165 hasConcept C49805395 @default.
- W3149933165 hasConcept C54355233 @default.
- W3149933165 hasConcept C86803240 @default.
- W3149933165 hasConcept C95444343 @default.
- W3149933165 hasConceptScore W3149933165C102230213 @default.
- W3149933165 hasConceptScore W3149933165C104317684 @default.
- W3149933165 hasConceptScore W3149933165C141035611 @default.
- W3149933165 hasConceptScore W3149933165C150194340 @default.
- W3149933165 hasConceptScore W3149933165C153911025 @default.
- W3149933165 hasConceptScore W3149933165C190062978 @default.
- W3149933165 hasConceptScore W3149933165C2776879804 @default.
- W3149933165 hasConceptScore W3149933165C49805395 @default.
- W3149933165 hasConceptScore W3149933165C54355233 @default.
- W3149933165 hasConceptScore W3149933165C86803240 @default.
- W3149933165 hasConceptScore W3149933165C95444343 @default.
- W3149933165 hasIssue "3" @default.
- W3149933165 hasLocation W31499331651 @default.
- W3149933165 hasOpenAccess W3149933165 @default.
- W3149933165 hasPrimaryLocation W31499331651 @default.
- W3149933165 hasRelatedWork W1537082411 @default.
- W3149933165 hasRelatedWork W1559147833 @default.
- W3149933165 hasRelatedWork W1951010197 @default.
- W3149933165 hasRelatedWork W1979694064 @default.
- W3149933165 hasRelatedWork W2010997845 @default.
- W3149933165 hasRelatedWork W2038849646 @default.
- W3149933165 hasRelatedWork W2064376337 @default.
- W3149933165 hasRelatedWork W2077986876 @default.
- W3149933165 hasRelatedWork W2086735902 @default.
- W3149933165 hasRelatedWork W2107406982 @default.
- W3149933165 hasRelatedWork W2156687140 @default.
- W3149933165 hasRelatedWork W2158693780 @default.
- W3149933165 hasRelatedWork W2168681690 @default.
- W3149933165 hasRelatedWork W2282593169 @default.
- W3149933165 hasRelatedWork W2396394629 @default.
- W3149933165 hasRelatedWork W2472256349 @default.
- W3149933165 hasRelatedWork W2503848032 @default.
- W3149933165 hasRelatedWork W2530924338 @default.
- W3149933165 hasRelatedWork W2604143792 @default.
- W3149933165 hasRelatedWork W3034475592 @default.
- W3149933165 isParatext "false" @default.
- W3149933165 isRetracted "false" @default.
- W3149933165 magId "3149933165" @default.
- W3149933165 workType "article" @default.