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- W3149979231 abstract "cDNA libraries from aborted human 3-month fetal brain, adult rat and mouse brain were constructed by using a XZAP express cDNA library construction kit. Low molecular weight fragments of the second strand cDNAs were removed by flowing through the Sepharose CL-4B column and the fractionated long, middle, short fragments and the combined fragments were respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus. The 5' ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced. A total of 894 ESTs were obtained and some full-length clones were sequenced. By analyzing the sequences, 12 novel full-length cDNAs were obtained." @default.
- W3149979231 created "2021-04-13" @default.
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- W3149979231 date "2002-01-01" @default.
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- W3149979231 title "Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs" @default.
- W3149979231 hasPublicationYear "2002" @default.
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