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- W3149991422 abstract "Introduction: The pear Pyrus boissieriana Buhse is a species of pear that belongs to the plant family Rosacea that grows in northern Iran and Turkmenistan.The pear leaves are useful for treatment of inflammation of bladder bacteriuria, high blood pressure and decrease blood sugar. Hep-G2 is a perpetual cell line which was derived from the liver tissue of a 15 year old Caucasian American male with a well differentiated hepatocellular carcinoma. HepG2 cells are a suitable in vitro model system for the study of polarized human hepatocytes. The purpose of this study was to examine the in vitro cytotoxicity of Pyrus boissieriana Buhse leaves extract on human hepatoblastoma HepG2 cells. Materials andmethods: In this study Hep-G2 cells growing in RPMI 1640 with 10% FBS for determination of the cytotoxicity measured by MTT assay. MTT assay is colorimetric assay for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, and WSTs) to formazan dyes, giving a purple color. Cells were cultured in 24 well plates. After cell treatment with different concentration of Pyrus boissieriana Buhse leaves extract, MTT assay was performed. Different concentrations such as 0.02%, 0.05%, 0.2%, 0.5%, 1%, 1.5% and 2% were tested. Results: We have seen different results from different concentrations. These findings show that Pyrus boissieriana Buhse leaves extract concentrations lower than 1% was noncytotoxic (P=0.037911). Most cytotoxicity was seen in 2% concentration and there is no difference between .02% and .05% concentrations in comparison with the control group." @default.
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- W3149991422 date "2011-01-01" @default.
- W3149991422 modified "2023-09-23" @default.
- W3149991422 title "Cytotoxicity of methylsulfonylmethane (MSM) on esophageal cancer cell line (KYSE30)" @default.
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