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- W3150244312 abstract "Microsclerotia (MS) formation was suc- cessfully induced in Nomuraea rileyi (Ascomycetes: Hypocreales) in liquid amended medium (AM) cul- ture. To improve MS production, the culture medium was varied. Three quantitative variables, carbon, nitrogen, and basal salt sources, were investigated using the AM. An orthogonal experiment (L9 (3) 3 ) design was applied to optimize the culture medium. Maximum MS production (21.9 9 10 4 MS ml -1 ) was obtained using the optimized culture medium (32.0 g l -1 of glucose, 2.0 g l -1 of ammonium citrate, and 0.15 g l -1 of ferrous sulfate). Subsequently, viability, including thermotolerance, shelf-life, and infection, were investigated to evaluate the potential of MS for use as a mycoinsecticide. The MS, collected from the optimized culture medium, exhibited viru- lence and greater thermotolerance than conidia, and maintained 86.4 % germinability after one-year room temperature storage. These results suggested that the MS obtained through optimization production can be used as a mycoinsecticide." @default.
- W3150244312 created "2021-04-13" @default.
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- W3150244312 date "2014-01-01" @default.
- W3150244312 modified "2023-09-24" @default.
- W3150244312 title "Optimization of culture medium for microsclerotia production by Nomuraea rileyi and analysis of their viability for use as a mycoinsecticide" @default.
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