Matches in SemOpenAlex for { <https://semopenalex.org/work/W3150247892> ?p ?o ?g. }
- W3150247892 abstract "Summary The coronavirus disease 2019 (COVID-19) pandemic, which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global public health challenge. While the efficacy of vaccines against emerging and future virus variants remains unclear, there is a need for therapeutics. Repurposing existing drugs represents a promising and potentially rapid opportunity to find novel antivirals against SARS-CoV-2. The virus encodes at least nine enzymatic activities that are potential drug targets. Here we have expressed, purified and developed enzymatic assays for SARS-CoV-2 nsp13 helicase, a viral replication protein that is essential for the coronavirus life cycle. We screened a custom chemical library of over 5000 previously characterised pharmaceuticals for nsp13 inhibitors using a FRET-based high-throughput screening (HTS) approach. From this, we have identified FPA-124 and several suramin-related compounds as novel inhibitors of nsp13 helicase activity in vitro . We describe the efficacy of these drugs using assays we developed to monitor SARS-CoV-2 growth in Vero E6 cells." @default.
- W3150247892 created "2021-04-13" @default.
- W3150247892 creator A5001640638 @default.
- W3150247892 creator A5008803665 @default.
- W3150247892 creator A5010187326 @default.
- W3150247892 creator A5013925603 @default.
- W3150247892 creator A5025058594 @default.
- W3150247892 creator A5029094962 @default.
- W3150247892 creator A5029333589 @default.
- W3150247892 creator A5029512529 @default.
- W3150247892 creator A5030207846 @default.
- W3150247892 creator A5030490991 @default.
- W3150247892 creator A5032591023 @default.
- W3150247892 creator A5040052939 @default.
- W3150247892 creator A5043267123 @default.
- W3150247892 creator A5058266138 @default.
- W3150247892 creator A5060445219 @default.
- W3150247892 creator A5065145095 @default.
- W3150247892 creator A5071095963 @default.
- W3150247892 creator A5071625312 @default.
- W3150247892 date "2021-04-08" @default.
- W3150247892 modified "2023-10-14" @default.
- W3150247892 title "Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp13 Helicase" @default.
- W3150247892 cites W1965280032 @default.
- W3150247892 cites W1967006578 @default.
- W3150247892 cites W1988135057 @default.
- W3150247892 cites W1991001144 @default.
- W3150247892 cites W1991049232 @default.
- W3150247892 cites W1995042237 @default.
- W3150247892 cites W2001130038 @default.
- W3150247892 cites W2003116453 @default.
- W3150247892 cites W2004610587 @default.
- W3150247892 cites W2020347526 @default.
- W3150247892 cites W2020629025 @default.
- W3150247892 cites W2023450351 @default.
- W3150247892 cites W2030429617 @default.
- W3150247892 cites W2037978116 @default.
- W3150247892 cites W2039384791 @default.
- W3150247892 cites W2061061337 @default.
- W3150247892 cites W2062941476 @default.
- W3150247892 cites W2065496099 @default.
- W3150247892 cites W2078510009 @default.
- W3150247892 cites W2084620659 @default.
- W3150247892 cites W2084710095 @default.
- W3150247892 cites W2112277337 @default.
- W3150247892 cites W2112898697 @default.
- W3150247892 cites W2127287358 @default.
- W3150247892 cites W2128581376 @default.
- W3150247892 cites W2140142022 @default.
- W3150247892 cites W2146216242 @default.
- W3150247892 cites W2146900701 @default.
- W3150247892 cites W2150409982 @default.
- W3150247892 cites W2151877638 @default.
- W3150247892 cites W2151920397 @default.
- W3150247892 cites W2154183412 @default.
- W3150247892 cites W2239581473 @default.
- W3150247892 cites W2292021561 @default.
- W3150247892 cites W2303909027 @default.
- W3150247892 cites W2344664973 @default.
- W3150247892 cites W2463036497 @default.
- W3150247892 cites W2589174989 @default.
- W3150247892 cites W2592411747 @default.
- W3150247892 cites W2683350484 @default.
- W3150247892 cites W2724799126 @default.
- W3150247892 cites W2794096509 @default.
- W3150247892 cites W2886709672 @default.
- W3150247892 cites W2939293293 @default.
- W3150247892 cites W2991491848 @default.
- W3150247892 cites W2995552435 @default.
- W3150247892 cites W3004896487 @default.
- W3150247892 cites W3005212621 @default.
- W3150247892 cites W3017680297 @default.
- W3150247892 cites W3020188044 @default.
- W3150247892 cites W3022285953 @default.
- W3150247892 cites W3022916237 @default.
- W3150247892 cites W3027630905 @default.
- W3150247892 cites W3033960862 @default.
- W3150247892 cites W3043431476 @default.
- W3150247892 cites W3045645692 @default.
- W3150247892 cites W3092024794 @default.
- W3150247892 cites W3094782071 @default.
- W3150247892 cites W3095556560 @default.
- W3150247892 cites W3096064145 @default.
- W3150247892 cites W3096967708 @default.
- W3150247892 cites W3117730334 @default.
- W3150247892 cites W3119203274 @default.
- W3150247892 doi "https://doi.org/10.1101/2021.04.07.438808" @default.
- W3150247892 hasPublicationYear "2021" @default.
- W3150247892 type Work @default.
- W3150247892 sameAs 3150247892 @default.
- W3150247892 citedByCount "1" @default.
- W3150247892 countsByYear W31502478922021 @default.
- W3150247892 crossrefType "posted-content" @default.
- W3150247892 hasAuthorship W3150247892A5001640638 @default.
- W3150247892 hasAuthorship W3150247892A5008803665 @default.
- W3150247892 hasAuthorship W3150247892A5010187326 @default.
- W3150247892 hasAuthorship W3150247892A5013925603 @default.
- W3150247892 hasAuthorship W3150247892A5025058594 @default.
- W3150247892 hasAuthorship W3150247892A5029094962 @default.
- W3150247892 hasAuthorship W3150247892A5029333589 @default.