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- W3150279970 abstract "Optical microscopy plays an essential role in biological studies due to its capability and compatibility of non-contact, minimally invasive observation and mea- surement of live specimens. However, the conventional optical microscopy only has a spatial resolution about 200 nm due to the Abbe diffraction limit, and also lacks the ability of three-dimensional imaging. Super-resolution far- field optical microscopy based on special illumination schemes has been dramatically developed over the last decade. Among them, only the structured illumination microscopy (SIM) is of wide-field geometry that enables it easily compatible with the conventional optical micro- scope. In this article, the principle of SIM was introduced in terms of point spread function (PSF) and optical trans- form function (OTF) of the optical system. The SIM for super-resolution (SIM-SR) proposed by Gustafsson et al. and the SIM for optical sectioning (SIM-OS) pro- posed by Neil et al. are the most popular ones in the research community of microscopy. They have the same optical configuration, but with different data post- processing algorithms. We mathematically described the basic theories for both of the SIMs, respectively, and pre- sented some numerical simulations to show the effects of super-resolution and optical sectioning. Various approaches to generation of structured illumination patterns were reviewed. As an example, a SIM system based on DMD- modulation and LED-illumination was demonstrated. A lateral resolution of 90 nm was achieved with gold nano- particles. The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain neurons, and the sectioning strength of 930 nm was obtained." @default.
- W3150279970 created "2021-04-13" @default.
- W3150279970 creator A5025336556 @default.
- W3150279970 creator A5044858745 @default.
- W3150279970 date "2014-01-01" @default.
- W3150279970 modified "2023-09-27" @default.
- W3150279970 title "Structured illumination microscopy for super-resolution and optical sectioning" @default.
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