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- W3150311667 abstract "Objective To construct single chain antibody specific to membrane protein of Schistosoma japonicum by gonetic engineering technique. Methods The V-H (heavy-chain variable region) and V-L (light-chain variable region) genes were amplified by PCR from the genomic DNA of NP11-4 cell line, and sequenced by Sanger's method. The ScFv was constructed in pTHA90 vector using V-H and V-L genes, then expressed by IPTG. Results The V-H and V-L genes were obtained through PCR. The DNA sequences showed that V-H and V-L were new variable region genes of antibody. They were registered by GenBank. A ScFv gene with (Gly4Ser) 3 intralinker in the pTHA90 vector was successfully constructed. The ScFv was expressed as thioredoxin-fused proteins about 36^2 kDa. Conclusion A specific ScFv against the membrane protein of Schistosoma japonicum was constructed and expressed." @default.
- W3150311667 created "2021-04-13" @default.
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- W3150311667 date "2001-01-01" @default.
- W3150311667 modified "2023-09-25" @default.
- W3150311667 title "Gene Cloning, Construction and Expression of Single-Chain Fv (scFv) Against the Membrane Protein of Schisotosoma japonicum" @default.
- W3150311667 hasPublicationYear "2001" @default.
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