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- W3150317191 abstract "双 shelled 草鲤鱼 reovirus (GCRV ) 能够内长的 RNA 抄写并且处理。染色体顺序分析表明了蛋白质 VP2，在基因片断 2 编码了(S2 ) ，是通常认为的 RNA 依赖的 RNA 聚合酶(RdRp ) 。在以前的工作，我们表示了与 RNA 聚合酶活动被联系的 VP2 的功能的区域(作为 rVP2390900 表示了) 在 E。coli 并且对 VP2 准备了 polyclonal 抗体。描绘 GCRV RNA 聚合酶， recombinant 全身的 VP2 (rVP2 ) 首先在一个 baculovirus 系统被构造并且表示，，有一个依附的他的标签的熔化蛋白质。Immunofluorescence (如果) 试金，和 immunoblot (IB ) ，从两个的分析表示了房间摘录并且净化了 Histagged rVP2，证明 rVP2 成功地在 Sf9 房间被表示。replicase 活动的进一步的描述显示出 poly 展出的那净化的 rVP2 和 GCRV 粒子(C) 依赖者 poly (G) 聚合酶活动。RNA 酶的活动要求了二价的阳离子 Mg2+ ，并且在 28 点是最佳的 ???????????? t" @default.
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- W3150317191 date "2014-01-01" @default.
- W3150317191 modified "2023-09-23" @default.
- W3150317191 title "The VP2 protein of grass carp reovirus （GCRV） expressed in a baculovirus exhibits RNA polymerase activity" @default.
- W3150317191 hasPublicationYear "2014" @default.
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