FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3150376427> ?p ?o ?g. }

• W3150376427 abstract "1074-Pos Board B829 Quantifying Short-Lived Events in Multi-State Ionic Channel Measurements Arvind Balijepalli1,2, Canute Vaz1, Jessica Ettedgui1, Andrew T. Cornio1, Joseph W.F. Robertson1, Kin P. Cheung1, Richard W. Pastor2, John J. Kasianowicz1. NIST, Gaithersburg, MD, USA, NIH, Rockville, MD, USA. We present a new technique that considerably improves the resolution and accuracy of single molecule measurements with nanopores. Molecular interactions with nanopores are characterized by electrical measurements of discrete changes in the channel conductance. By representing physical components of the system with electrical equivalents, we used circuit theory to model response of the system to a stimulus (e.g., a molecule entering the channel). This allowed us to characterize short-lived events where the ionic current does not reach a steady state value, and were previously not analyzed. Applying this technique to measurements of poly(ethylene glycol) (PEG) molecules with the a-hemolysin (aHL) nanopore resulted in remarkable improvements in accuracy and the number of detected events. When measuring polydisperse PEG (mean molecular weights of 400 g/mol and 600 g/mol), the new method recovered z 18-fold more events per unit time, compared with existing techniques, and discriminated molecules with as few as 8 monomers (PEG8). We validated the measurement of PEG with an aHL nanopore using results from a recently published study (Balijepalli et. al, J Am Chem Soc, 135: 7064, 2013) that refined a previous analytical theory with molecular dynamics simulations. Fitting this model to the newly obtained experimental data resulted in excellent agreement of both the blockade depth (the ratio of the ionic current when a molecule occupies the pore to the open channel current) and the residence times of the molecule in the channel, over the entire measurement range (PEG8 to PEG19). Finally, we applied the new analysis technique to recover the sequence of a known DNA strand with 26 bases, from a published ionic current trace (Manrao et. al, Nat. Biotechnol. 30: 349, 2012). The technique detected systematic fluctuations in the ionic current that were as small as 0.9 5 0.04 pA." @default.
• W3150376427 created "2021-04-13" @default.
• W3150376427 creator A5010641447 @default.
• W3150376427 creator A5016507629 @default.
• W3150376427 creator A5023990409 @default.
• W3150376427 creator A5024128533 @default.
• W3150376427 creator A5026244966 @default.
• W3150376427 creator A5029634228 @default.
• W3150376427 creator A5031468678 @default.
• W3150376427 creator A5032025883 @default.
• W3150376427 creator A5084407733 @default.
• W3150376427 date "2014-01-01" @default.
• W3150376427 modified "2023-09-23" @default.
• W3150376427 title "Osmotically-Driven Transport through Carbon Nanotube Pores" @default.
• W3150376427 hasPublicationYear "2014" @default.
• W3150376427 type Work @default.
• W3150376427 sameAs 3150376427 @default.
• W3150376427 citedByCount "0" @default.
• W3150376427 crossrefType "journal-article" @default.
• W3150376427 hasAuthorship W3150376427A5010641447 @default.
• W3150376427 hasAuthorship W3150376427A5016507629 @default.
• W3150376427 hasAuthorship W3150376427A5023990409 @default.
• W3150376427 hasAuthorship W3150376427A5024128533 @default.
• W3150376427 hasAuthorship W3150376427A5026244966 @default.
• W3150376427 hasAuthorship W3150376427A5029634228 @default.
• W3150376427 hasAuthorship W3150376427A5031468678 @default.
• W3150376427 hasAuthorship W3150376427A5032025883 @default.
• W3150376427 hasAuthorship W3150376427A5084407733 @default.
• W3150376427 hasConcept C113196181 @default.
• W3150376427 hasConcept C121332964 @default.
• W3150376427 hasConcept C121932024 @default.
• W3150376427 hasConcept C141795571 @default.
• W3150376427 hasConcept C145148216 @default.
• W3150376427 hasConcept C159467904 @default.
• W3150376427 hasConcept C171250308 @default.
• W3150376427 hasConcept C178790620 @default.
• W3150376427 hasConcept C185592680 @default.
• W3150376427 hasConcept C192562407 @default.
• W3150376427 hasConcept C2182769 @default.
• W3150376427 hasConcept C26873012 @default.
• W3150376427 hasConcept C2777516009 @default.
• W3150376427 hasConcept C32909587 @default.
• W3150376427 hasConcept C513720949 @default.
• W3150376427 hasConceptScore W3150376427C113196181 @default.
• W3150376427 hasConceptScore W3150376427C121332964 @default.
• W3150376427 hasConceptScore W3150376427C121932024 @default.
• W3150376427 hasConceptScore W3150376427C141795571 @default.
• W3150376427 hasConceptScore W3150376427C145148216 @default.
• W3150376427 hasConceptScore W3150376427C159467904 @default.
• W3150376427 hasConceptScore W3150376427C171250308 @default.
• W3150376427 hasConceptScore W3150376427C178790620 @default.
• W3150376427 hasConceptScore W3150376427C185592680 @default.
• W3150376427 hasConceptScore W3150376427C192562407 @default.
• W3150376427 hasConceptScore W3150376427C2182769 @default.
• W3150376427 hasConceptScore W3150376427C26873012 @default.
• W3150376427 hasConceptScore W3150376427C2777516009 @default.
• W3150376427 hasConceptScore W3150376427C32909587 @default.
• W3150376427 hasConceptScore W3150376427C513720949 @default.
• W3150376427 hasLocation W31503764271 @default.
• W3150376427 hasOpenAccess W3150376427 @default.
• W3150376427 hasPrimaryLocation W31503764271 @default.
• W3150376427 isParatext "false" @default.
• W3150376427 isRetracted "false" @default.
• W3150376427 magId "3150376427" @default.
• W3150376427 workType "article" @default.