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- W3150400150 abstract "The TCR BV gene repertoire in the human T cell lines specific for swine SLA were analyzed by semi-quantitative PCR and Genescan protocol with DNA sequencer. The highly restricted TCR Vbeta usage was observed from T cell lines in the human anti-porcine response system, suggesting that a limited number of xenogeneic epitopes were recognized by human T clones. Before priming, the member from all 24 Vbeta gene families except BV24, could be identified to be used. After multiple repeatedly stimulation with porcine PBMC, however, the usage pattern of Vbeta gene segments in the T cell lines changed dramatically. Two CD4~(+) T cell lines shared BV12 and BV13, one of CD4~(+) T cell lines preferred to express BV12 (usage frequency 79.9%). Moreover, there were common usage of BV1 in three of the CD8~(+) T cell lines with a highest usage frequency 52.7 percent in the cell line G. TCR Vbeta uage patterns within CD8~(+) T cell lines seemed different from those expressed by CD4~(+) T cells, a fact consistent with the observation that CD8~(+) T cells and CD4~(+) T cells responded to SLA in different manner, probably by a differential recognition of MHC/peptide complexes." @default.
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- W3150400150 date "2001-01-01" @default.
- W3150400150 modified "2023-10-17" @default.
- W3150400150 title "The T cell receptor BV gene usage patterns and the clonal characterisation of CDR3 in the xenogeneic human anti-swine T cell lines" @default.
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