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- W3150402570 abstract "Heparin inhibits arterial smooth muscle cell (SMC) proliferation in vivo and in vitro; moreover, it reinduces the expression of alpha-smooth muscle (SM) actin (an accepted marker of SMC differentiation) in SMCs of the intimal thickening that develops after experimentally induced endothelial lesions. We have investigated the effect of heparin on the proliferation and actin isoform expression in cultured rat SMCs. In the presence of 10% fetal calf serum (FCS), heparin-treated primary and passage 5 SMCs showed a decrease of proliferation and an increase of alpha-SM actin (measured by Western blots or two-dimensional gel electrophoresis) compared with untreated SMCs. When SMCs were cultured in the presence of 10% plasma-derived serum, no proliferation occurred and heparin did not modify alpha-SM actin expression. This suggests that the action of heparin is related to its antiproliferative activity. SMCs cultured in the presence of 10% FCS plus heparin had the same level of proliferation as SMCs cultured in 5%..." @default.
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- W3150402570 date "1991-01-01" @default.
- W3150402570 modified "2023-09-24" @default.
- W3150402570 title "Modulation of actin isoform expression in cultured arterial smooth muscle cells by heparin and culture conditions." @default.
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