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- W3150505338 abstract "Acetaldehyde,salsolinol(Sal) and 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-β-carboline(6-OHMTHβC) in neonatal rat brain after acute ethanol treatment were determined by high performance liquid chromatography.Free acetaldehyde in rat brain homogenate was derivatized with 2,4-dinitrophenylhydrazine(2,4DNPH) at 60 ℃ for 15 min.The derivate product was separated on an ODS-C18 column(4.6 mm × 150 mm) and detected at 360 nm.The method was proved to be simple,sensitive and reliable.In comparison with the control,analysis of neonatal rat brain samples revealed a significant difference in the level of acetaldehyde within 24 h of 5.0 g/kg ethanol treatment(P 0.05).The determination of Sal and 6-OH-MTHβC by HPLC was performed on a HS F5 column(250 mm × 4.6 mm),with a Coularray Detector-4 as the electrochemical detector.The results indicated that the levels of Sal and 6-OH-MTHβC in rat brain increased significantly with the elevated level of acetaldehyde after acute alcohol administration.The condensation of acetaldehyde with monoamine neurotransmitters may play an important role in central nervous system dysfunction after alcoholism." @default.
- W3150505338 created "2021-04-13" @default.
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- W3150505338 date "2010-01-01" @default.
- W3150505338 modified "2023-09-25" @default.
- W3150505338 title "Determination of Acetaldehyde,Salsolinol and 6-Hydroxy-1-methyl1,2,3,4-tetrahydro-β-carboline in Brains after Acute Ethanol Administration to Neonatal Rats" @default.
- W3150505338 hasPublicationYear "2010" @default.
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