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- W3150508614 endingPage "339" @default.
- W3150508614 startingPage "325" @default.
- W3150508614 abstract "Abstract Thermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and locations. Eight compost piles and ten soil sites were sampled in combination with different culture-dependent approaches to describe suitable strategies for the isolation and selection of thermophilous fungi. Additionally, an approach with a heat-shock treatment, but without elevated temperature incubation led to the isolation of heat-resistant mesophilic species. The cultures were identified based on morphology, DNA barcodes, and microsatellite fingerprinting. In total, 191 obtained isolates were assigned to 31 fungal species, from which half are truly thermophilic or thermotolerant, while the other half are heat-resistant fungi. A numerous amount of heat-adapted fungi was isolated from both compost and soil samples, indicating the suitability of the used approaches and that the richness and availability of those organisms in such environments are substantially high." @default.
- W3150508614 created "2021-04-13" @default.
- W3150508614 creator A5021394489 @default.
- W3150508614 creator A5049686464 @default.
- W3150508614 creator A5054943193 @default.
- W3150508614 date "2021-03-01" @default.
- W3150508614 modified "2023-10-02" @default.
- W3150508614 title "Improved strategies to efficiently isolate thermophilic, thermotolerant, and heat-resistant fungi from compost and soil" @default.
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