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- W3150662506 abstract "One solid-state electrochemiluminescence(ECL) protein biosensor based the competing reaction and substitute reaction between protein-to-DNA aptamer and DNA-to-DNA aptamer was proposed.Additionally,the biosensor was based ECL photo-quenching effect of ferrocene(Fc) to tris(2,2'-bipyridyl)ruthenium(II)(Ru(bpy)32+).It was built up by modification of Au nanoparticles(AuNPs) and Ru(bpy)3 2+ one Au electrode firstly,and then self-assembly of one special double-stranded DNA(dsDNA) onto the electrode.This dsDNA was prepared by hybridization of one Fc labeled molecular beacon single-stranded DNA(ssDNA) and one anti-thrombin aptamer ssDNA.Without the target protein,this Fc-dsDNA/Ru(bpy)3 2+-AuNPs/Au electrode trigged strong ECL signal,so we called it ECL on state.When thrombin was present in the sensing solution,the protein reacted with its aptamer from the Fc-dsDNA/Ru(bpy)3 2+-AuNPs/Au electrode.Then the left molecular beacon ssDNA the electrode recovered to its normal stem-loop structure and consequently its Fc labeler was close enough to the electrode surface to quench the ECL signal from Ru(bpy)3 2+.It was in ECL off state.We measured the decrease in ECL intensity to sense the target protein.This was one endeavour to sense protein by using un-labeling target or probe strategy,which gave higher sensitivity and selectivity due to the better combination efficiency of protein and the un-labeled aptamer.6.25 fmol/L thrombin was detected out." @default.
- W3150662506 created "2021-04-13" @default.
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- W3150662506 date "2011-01-01" @default.
- W3150662506 modified "2023-09-22" @default.
- W3150662506 title "Solid-state electrochemiluminescence protein biosensor with aptamer substitution strategy" @default.
- W3150662506 hasPublicationYear "2011" @default.
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