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- W3150668022 abstract "Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFNα1 and staphylokinase secreted from Bacillus subtilis GB500 cells. Both proteins were present in the cytoplasma and the cell enveloped pointing to a posttranslational mode of translocation across the cytoplasmic membrane. 5- to 10-fold higher concentrations of gold particles per 0.1 μm2 were found on the cell enveloped and clustering was observed suggesting preferential regions for secretion sites. Several control experiments ensured the specificity of the labelling data." @default.
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- W3150668022 date "1989-12-01" @default.
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- W3150668022 title "Immunoelectron microscopy ofBacillus subtiliscells secreting human interferon α1or staphylokinase" @default.
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- W3150668022 doi "https://doi.org/10.1111/j.1574-6968.1989.tb03683.x" @default.
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