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• W3150789166 abstract "Full Length Research Article The challenges of morphologic variations in Clarias gariepinus without established data on their biochemical and genotypic relationships necessitated the use of molecular tool in establishing their biochemical and phylogenetic relationships. This study investigated the relationship in two (2) pectoral spine variant groups in C. gariepinus using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Protein extraction and gel electrophoresis of blood samples from 18 individuals of the species obtained from a dam system were subjected to extraction buffer and SDS PAGE at 12.5%. Variants were biochemically compared based on produced phenogram. Biochemical markers were established by comparing molecular weight of phena-specific bands with standard protein using kDa ladder. Phylogenetic relationship and subsequent test for correctness of phenotypic grouping was established via cluster analysis of scored profiles and Canonical classification, respectively. The produced phenogram revealed that the variants can be separated by a low molecular weight biochemical marker which corresponds with α-amylase inhibitor. Dendrogram generated 8 clusters at 0.63 coefficient of variation. 75% members of one of the groups clustered while canonical classification revealed 100% correctness of original phenotypic grouping. It was concluded that the variants were biochemically and genetically discriminant varieties of C. gariepinus. It was also concluded that this information would be of use in taxa discrimination and marker assisted selection for stock improvement in the species while differential biochemical characteristics of the sub-groups would have nutritional and medical importance." @default.
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• W3150789166 date "2013-01-01" @default.
• W3150789166 modified "2023-09-26" @default.
• W3150789166 title "Genetic and biochemical differentiation of pectoral spine variants in Clarias gariepinus" @default.
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