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- W3150874476 abstract "An electrochemical immunosensor for rapid detection of alphafetoprotein(AFP) was proposed.Polypyrrole(PPy) was firstly synthesized on the surface of expanded graphite(EG) electrode by potentiostatic method,and then horseradish peroxidase(HRP) labeled AFP-antibody(HRP-anti-AFP) was fixed on the immunosensor interface by using glutaraldehyde(GA) as coupling reagent.After incubation of the immunosensor in the solution containing AFP sample at 35 ℃ for 50 min,Prussian blue(PB) was immobilized onto the immunosensor interface as electron mediator.The immune complexes produced by the combination of antibody and antigen reduced the oxidation peak current of PB.Under optimized experimental conditions,AFP was detected in a concentration range from 0.01 to 300 μg/L and a detection limit of 6.25 ng/L(S/N=3) was obtained.Considering its simplicity,high sensitivity and low cost,the immunosensor based on PPy modified EG electrode is feasible to be developed as a disposable electrochemical sensor for clinical purposes." @default.
- W3150874476 created "2021-04-13" @default.
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- W3150874476 date "2013-01-01" @default.
- W3150874476 modified "2023-09-23" @default.
- W3150874476 title "Electrochemical Immunosensor for Detection of Alphafetoprotein Based on Polypyrrole Modified Expanded Graphite Electrode" @default.
- W3150874476 hasPublicationYear "2013" @default.
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