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- W3150878035 abstract "Objective To provide a more reasonable regimen of temozolomide and thalidomide, and study the mechanism of these 2 drugs in inhibiting the proliferation and growth of U251 glioma cell in vitro.Methods Human glioma cell line U251 was cultured in vitro and divided into different treatment groups for 3 d:temozolomide group(100μmol/L),thalidomide group(100μg/L),temozolomide(100μmol/L) with thalidomide group(100μg/L)and vehicle control group.After different treatment for 3 d,3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyhetrazolium bromide(MTT)was adopted for the determination of cell viability,and cell cycle was analysed by flow cytometry.After labeled with acridine orange(AO), autophagy vesicles were quantitatively detected by flow cytometry.TdT-mediated dUTP-biotin nick end labeling(TUNEL)was employed in observing and detecting the apoptosis of treated cells.Western blotting was used in examining the autophagy and apoptosis-related proteins.Results Compared with the 2 drugs used alone,temozolomide with thalidomide imposed more obvious inhibition on tumor cell growth(P= 0.000,for all).Combination of 2 drugs induced tumor cell cycle arrest in G_0-G_1.Both of autophagic and apoptotic cell death could be induced by temozolomide with thalidomide in U251.In two-drug treatment group,the expression of autophagy-related microtubule-associated protein 1 light chain 3(MAP1LC3)and apoptosis-related Caspase-3 was significantly higher in transcriptional level in comparing with single drug treatment group(P=0.000,for all).Conclusion Temozolomide combined with thalidomide may induce 2 types of programmed cell death—apoptotic and autophagic cell death by up-regulating the expression of related genes in U251 glioma cell,thereby,the combination of these 2 drugs may suppress the proliferation and growth of U251 glioma cell in vitro." @default.
- W3150878035 created "2021-04-13" @default.
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- W3150878035 date "2010-01-01" @default.
- W3150878035 modified "2023-09-24" @default.
- W3150878035 title "Study on mechanism of thalidomide combined with temozolomide to suppress proliferation of U251 glioma cell in vitro" @default.
- W3150878035 hasPublicationYear "2010" @default.
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