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- W3150878621 abstract "Objective:To investigate the morphological characteristics of the cultured antigen-loaded DC of mice in vitro. It will provide morphological basis for the tumor biological therapy. Methods:DCs were separated and cultured,tumor antigen was extracted from B16 melanoma cells by method of freeze thawing,and then they were co-cultured. The co-cultured cells were antigen-loaded DC. The mouse model of B16 melanoma was established,and antigen-loaded DCs were injected into the tumor-surrounding tissues. The morphology of antigen-loaded DCs was observed by optical microscopy,electron microscopy and immunohisochemistry. Results:The antigen-loaded DC cells were bigger than the DC cells,and protrusions on the surface of antigen-loaded DC were more grossus and curved. The antigen-loaded DC cells mainly distributed in the tumor-surrounding tissues and regional lymph nodes by immunohisochemistry. Under electron microscopy,the antigen-loaded DC cells were bigger than the DC cells and notch always appears on cellular nucleus was observed. The protrusions on the surface of antigen-loaded DC cells contacted closely with tumor cells or lymphocytes. Conclusion:The morphological characteristics of antigen-loaded DC on function were more active than DC. These results suggested that the antigen-loaded DC was a good vaccine." @default.
- W3150878621 created "2021-04-13" @default.
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- W3150878621 date "2009-01-01" @default.
- W3150878621 modified "2023-09-23" @default.
- W3150878621 title "Morphological Characteristics of the Cultured Antigen-loaded DC of Mice in Vitro" @default.
- W3150878621 hasPublicationYear "2009" @default.
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