FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3150979114> ?p ?o ?g. }

• W3150979114 abstract "Purpose: TNF-α is a pro-inflammatory cytokine believed to be an important mediator of inflammatory bowel disease (IBD). TNF is known to induce cell shedding and increase permeability of the small intestine. We hypothesize that TNF induced intestinal cell shedding contributes to barrier dysfunction and increased antigen presentation. In this study, we used confocal laser endomicroscopy (CLE) and light microscopy (LM), to quantitate TNF induced epithelial cell shedding in the small intestine, and correlated cell shedding with dextran absorption and translocation of commensal bacteria. Methods: 129 Sv/Ev mice were divided into two groups: the treatment group received intraperitoneal injection of TNF-α (0.175μg/ g), while the control group received an equivalent volume of normal saline injection. CLE were performed using Optiscan confocal endomicroscope on exteriorized intestine stained with acriflavine. Epithelial cell shedding was quantitated using density of epithelial gaps created by shedding of intestinal cells. Epithelial density was defined as the total number of epithelial gaps per 1000 cells counted in 5-10 villi per mouse. For LM, frozen sections of the intestine were stained with alcian blue and nuclear fast red. 3D reconstructed CLE images and 2D LM sections were manually counted for epithelial gaps and cells. For FITCdextran absorption study, mice were gavaged with 0.6 mg/g dextran after an overnight fast, blood samples were collected after 4 hr for serum FITC-dextran determination. For translocation studies, mice were gavaged after an overnight fast with 10^10 GFP labelled E. coli JM 109 suspended in 0.17mL LB broth. After 24 hours, liver, spleen and blood samples were obtained for culture. Results: For saline and TNF treated mice (n=6), the CLEdetermined epithelial gap density (mean+SD) was 10.6 ± 2.4 and 47.1 ± 7.4 gaps/1000 cells (P = 0.001), respectively. The LM-determined epithelial gap density was 34.8 ± 6.0 and 64.2 ± 7.4 gaps/1000 cells (P <0.0001), respectively. The discrepancy in the gap density observed between CLE and LM were due to the 3D and 2D nature of image analysis. Serum dextran concentration in the saline and TNF treated mice were 0.59 ± 0.05, and 3.3 ± 0.5 μg/mL (P <0.001), respectively. The liver, spleen and blood cultures from the saline treated mice were all negative, while the TNF treated mice grew 1.4 ± 0.94 E 3 and 1.1 ± 0.5 E 3 cfu/mL of GFP labelled E. colifrom liver and spleen cultures, confirming translocation of commensal bacteria. Conclusion: TNF induced epithelial cell shedding in the small intestine as observed by significant increases in the epithelial gap density of 129 Sv/Ev mice using CLE and LM. This increase in gap density was associated with increased permeability to macromolecules as measured by dextran absorption and translocation of commensal bacteria." @default.
• W3150979114 created "2021-04-13" @default.
• W3150979114 creator A5018797184 @default.
• W3150979114 creator A5019736946 @default.
• W3150979114 creator A5021559660 @default.
• W3150979114 creator A5029626548 @default.
• W3150979114 creator A5055412375 @default.
• W3150979114 creator A5073453608 @default.
• W3150979114 creator A5078380336 @default.
• W3150979114 creator A5086421936 @default.
• W3150979114 date "2011-01-01" @default.
• W3150979114 modified "2023-10-17" @default.
• W3150979114 title "Overexpression of PepT1 Increases the Susceptibility of Mice to Colitis in a Process Involving NOD2 Activity" @default.
• W3150979114 hasPublicationYear "2011" @default.
• W3150979114 type Work @default.
• W3150979114 sameAs 3150979114 @default.
• W3150979114 citedByCount "0" @default.
• W3150979114 crossrefType "journal-article" @default.
• W3150979114 hasAuthorship W3150979114A5018797184 @default.
• W3150979114 hasAuthorship W3150979114A5019736946 @default.
• W3150979114 hasAuthorship W3150979114A5021559660 @default.
• W3150979114 hasAuthorship W3150979114A5029626548 @default.
• W3150979114 hasAuthorship W3150979114A5055412375 @default.
• W3150979114 hasAuthorship W3150979114A5073453608 @default.
• W3150979114 hasAuthorship W3150979114A5078380336 @default.
• W3150979114 hasAuthorship W3150979114A5086421936 @default.
• W3150979114 hasConcept C126322002 @default.
• W3150979114 hasConcept C134018914 @default.
• W3150979114 hasConcept C136009344 @default.
• W3150979114 hasConcept C142724271 @default.
• W3150979114 hasConcept C152605822 @default.
• W3150979114 hasConcept C153911025 @default.
• W3150979114 hasConcept C17991360 @default.
• W3150979114 hasConcept C185592680 @default.
• W3150979114 hasConcept C203014093 @default.
• W3150979114 hasConcept C2524010 @default.
• W3150979114 hasConcept C2775862500 @default.
• W3150979114 hasConcept C2776914184 @default.
• W3150979114 hasConcept C2777310793 @default.
• W3150979114 hasConcept C2777339698 @default.
• W3150979114 hasConcept C2777882243 @default.
• W3150979114 hasConcept C2778690821 @default.
• W3150979114 hasConcept C3019368612 @default.
• W3150979114 hasConcept C33923547 @default.
• W3150979114 hasConcept C529295009 @default.
• W3150979114 hasConcept C55493867 @default.
• W3150979114 hasConcept C71924100 @default.
• W3150979114 hasConcept C86803240 @default.
• W3150979114 hasConcept C8891405 @default.
• W3150979114 hasConceptScore W3150979114C126322002 @default.
• W3150979114 hasConceptScore W3150979114C134018914 @default.
• W3150979114 hasConceptScore W3150979114C136009344 @default.
• W3150979114 hasConceptScore W3150979114C142724271 @default.
• W3150979114 hasConceptScore W3150979114C152605822 @default.
• W3150979114 hasConceptScore W3150979114C153911025 @default.
• W3150979114 hasConceptScore W3150979114C17991360 @default.
• W3150979114 hasConceptScore W3150979114C185592680 @default.
• W3150979114 hasConceptScore W3150979114C203014093 @default.
• W3150979114 hasConceptScore W3150979114C2524010 @default.
• W3150979114 hasConceptScore W3150979114C2775862500 @default.
• W3150979114 hasConceptScore W3150979114C2776914184 @default.
• W3150979114 hasConceptScore W3150979114C2777310793 @default.
• W3150979114 hasConceptScore W3150979114C2777339698 @default.
• W3150979114 hasConceptScore W3150979114C2777882243 @default.
• W3150979114 hasConceptScore W3150979114C2778690821 @default.
• W3150979114 hasConceptScore W3150979114C3019368612 @default.
• W3150979114 hasConceptScore W3150979114C33923547 @default.
• W3150979114 hasConceptScore W3150979114C529295009 @default.
• W3150979114 hasConceptScore W3150979114C55493867 @default.
• W3150979114 hasConceptScore W3150979114C71924100 @default.
• W3150979114 hasConceptScore W3150979114C86803240 @default.
• W3150979114 hasConceptScore W3150979114C8891405 @default.
• W3150979114 hasLocation W31509791141 @default.
• W3150979114 hasOpenAccess W3150979114 @default.
• W3150979114 hasPrimaryLocation W31509791141 @default.
• W3150979114 hasRelatedWork W125191604 @default.
• W3150979114 hasRelatedWork W2065992503 @default.
• W3150979114 hasRelatedWork W2347590573 @default.
• W3150979114 hasRelatedWork W2348135543 @default.
• W3150979114 hasRelatedWork W2350549154 @default.
• W3150979114 hasRelatedWork W2351188790 @default.
• W3150979114 hasRelatedWork W2354112357 @default.
• W3150979114 hasRelatedWork W2374973244 @default.
• W3150979114 hasRelatedWork W2379958079 @default.
• W3150979114 hasRelatedWork W2384047366 @default.
• W3150979114 hasRelatedWork W2388854198 @default.
• W3150979114 hasRelatedWork W2394214334 @default.
• W3150979114 hasRelatedWork W2397424378 @default.
• W3150979114 hasRelatedWork W2418691847 @default.
• W3150979114 hasRelatedWork W2998543251 @default.
• W3150979114 hasRelatedWork W3004186563 @default.
• W3150979114 hasRelatedWork W3013318879 @default.
• W3150979114 hasRelatedWork W3029672411 @default.
• W3150979114 hasRelatedWork W3032796136 @default.
• W3150979114 hasRelatedWork W169406307 @default.
• W3150979114 isParatext "false" @default.
• W3150979114 isRetracted "false" @default.
• W3150979114 magId "3150979114" @default.
• W3150979114 workType "article" @default.