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- W3151171934 abstract "AP2/ERF transcription factors widely exist in plants,and participate in various biotic and abiotic stress responses. In order to improve cotton resistance to disease and abiotic stress,the expression vector containing Hh ERF2 and Pe DREB2 a transcription factor genes, which were isolated from resistant excellent psammophyte Halimodendron halodendron and Populus euphratica respectively,and driven by rd29 A promoter,was constructed and transferred into cotton( Gossypium hirsutum) by pollen-tube pathway transformation technology. After the transgenic cottons were treated with stress,the ectopic expression Hh ERF2 and Pe DREB2 a genes,and some downstream relative pathogenesis-related( PR) genes were analyzed by Real-time PCR in transgenic cotton. The results showed that theexpression of Hh ERF2 gene could be induced by Verticillium dahliae( Vd),drought and salt,while Pe DREB2 a could only be induced by drought and salt. Inoculated by Vd,the expression of some downstream relative PR genes and the enzyme activity that related to phenolic metabolism such as: PAL,SOD and POD all increased in the transgenic lines at the same times. The resistance to Vd was also enhanced in transgenic cotton. Analysis of physiological and biochemical characteristics under drought and high-salt stresses,which indicated that compared with the contrast,the soluble carbohydrates and relative water concentration in the leaves of transgenic cotton were significantly elevated,while the conductivity and malondialdehyde( MDA) content decreased significantly. These results demonstrated that transgenic cotton performed excellent tolerability to Vd,drought and high-salt stresses." @default.
- W3151171934 created "2021-04-13" @default.
- W3151171934 creator A5031644464 @default.
- W3151171934 date "2015-01-01" @default.
- W3151171934 modified "2023-09-25" @default.
- W3151171934 title "Analysis of Stress Resistance Ability of Transgenic Cotton Expressing HhERF2 and PeDREB2a Genes" @default.
- W3151171934 hasPublicationYear "2015" @default.
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