Matches in SemOpenAlex for { <https://semopenalex.org/work/W3151363906> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W3151363906 abstract "OBJECTIVE To explore the effect of total alkaloid from Fritillaria thunbergii(TAF) on reversing multidrug resistance(MDR) of human lung adenocarcinoma A549 /DDP cells.METHODS ① In vitro Cytoxicity and proliferation inhibitory rate of TAF(12.5-200 mg·L-1) was assessed by MTT method.TAF 9 mg·L-1 was used in subsequent reversal experiments.Cyclosporine A(Cys A) 1 mg·L-1 and tetrandrine(Tet) 1 mg·L-1 acted as positive control group.The amount of MDR1 mRNA and P-glycoprotein(P-gp) of A549 /DDP cells was measured by real time polymerase chain reaction and Western blotting,respectively.② In vivo BALB /c nude mice were used to establish an A549 /DDP tumor model.The mice were randomly divided into vehicle,DDP 5 mg·kg-1,TAF 2 mg·kg-1,DDP 5 mg·kg-1 plus TAF 0.5,1 and 2 mg·kg-1 groups.DDP was ip given every two days and TAF was ig given once a day.Tumor volume was measured every four days and tumor mass was detected after 13 d.RESULTS After incubation with TAF for 72 h,IC50 of TAF to A549 and A549 /DDP cells was 141 ± 5 and(298 ± 22) mg·L-1,respectively,and IC10 of TAF to A549 and A549 /DDP cells was 15.3 ± 1.9 and(9.0 ± 1.2) mg·L-1.IC50 of DDP without or with TAF 9 mg·L-1 on A549 /DDP cells was 14.06 ± 3.72 and(0.79 ± 0.14) mg·L-1,respectively,while there was no significant change in IC50 of A549 cells.TAF reversed DDP resistance of A549 /DDP cells with fold-reversal 17.80,which was higher than that of Cys A(10.16) and Tet(14.05).Compared with A549 /DDP cell vehicle group,MDR1 mRNA and P-gp expression in A549 /DDP cells was decreased by TAF(P 0.01).The tumor inhibitory rate of DDP 5 mg·kg-1 in vivo was 49.9%.The combination of DDP 5 mg·kg-1 and TAF 2 mg·kg-1 increased the tumor inhibition rate to 67.4%.CONCLUSION TAF enhances MDR reversal effect of DDP on A549 /DDP cells in vitro and in vivo,and down-regulates MDR1 mRNA and P-gp expression in A549 / DDP cells." @default.
- W3151363906 created "2021-04-13" @default.
- W3151363906 creator A5024756555 @default.
- W3151363906 date "2013-01-01" @default.
- W3151363906 modified "2023-09-28" @default.
- W3151363906 title "M ultidrug resistance reversal activity of total alkaloid from Fritillaria thunbergii on cisplatin-resistant human lung adenocarcinom a A549/DDP cells" @default.
- W3151363906 hasPublicationYear "2013" @default.
- W3151363906 type Work @default.
- W3151363906 sameAs 3151363906 @default.
- W3151363906 citedByCount "0" @default.
- W3151363906 crossrefType "journal-article" @default.
- W3151363906 hasAuthorship W3151363906A5024756555 @default.
- W3151363906 hasConcept C109316439 @default.
- W3151363906 hasConcept C133936738 @default.
- W3151363906 hasConcept C150903083 @default.
- W3151363906 hasConcept C153911025 @default.
- W3151363906 hasConcept C185592680 @default.
- W3151363906 hasConcept C202751555 @default.
- W3151363906 hasConcept C207001950 @default.
- W3151363906 hasConcept C2776694085 @default.
- W3151363906 hasConcept C2777667214 @default.
- W3151363906 hasConcept C2777752497 @default.
- W3151363906 hasConcept C2778239845 @default.
- W3151363906 hasConcept C2778707650 @default.
- W3151363906 hasConcept C2779934757 @default.
- W3151363906 hasConcept C2780783641 @default.
- W3151363906 hasConcept C501593827 @default.
- W3151363906 hasConcept C54355233 @default.
- W3151363906 hasConcept C55493867 @default.
- W3151363906 hasConcept C71240020 @default.
- W3151363906 hasConcept C81729549 @default.
- W3151363906 hasConcept C86803240 @default.
- W3151363906 hasConcept C98274493 @default.
- W3151363906 hasConceptScore W3151363906C109316439 @default.
- W3151363906 hasConceptScore W3151363906C133936738 @default.
- W3151363906 hasConceptScore W3151363906C150903083 @default.
- W3151363906 hasConceptScore W3151363906C153911025 @default.
- W3151363906 hasConceptScore W3151363906C185592680 @default.
- W3151363906 hasConceptScore W3151363906C202751555 @default.
- W3151363906 hasConceptScore W3151363906C207001950 @default.
- W3151363906 hasConceptScore W3151363906C2776694085 @default.
- W3151363906 hasConceptScore W3151363906C2777667214 @default.
- W3151363906 hasConceptScore W3151363906C2777752497 @default.
- W3151363906 hasConceptScore W3151363906C2778239845 @default.
- W3151363906 hasConceptScore W3151363906C2778707650 @default.
- W3151363906 hasConceptScore W3151363906C2779934757 @default.
- W3151363906 hasConceptScore W3151363906C2780783641 @default.
- W3151363906 hasConceptScore W3151363906C501593827 @default.
- W3151363906 hasConceptScore W3151363906C54355233 @default.
- W3151363906 hasConceptScore W3151363906C55493867 @default.
- W3151363906 hasConceptScore W3151363906C71240020 @default.
- W3151363906 hasConceptScore W3151363906C81729549 @default.
- W3151363906 hasConceptScore W3151363906C86803240 @default.
- W3151363906 hasConceptScore W3151363906C98274493 @default.
- W3151363906 hasLocation W31513639061 @default.
- W3151363906 hasOpenAccess W3151363906 @default.
- W3151363906 hasPrimaryLocation W31513639061 @default.
- W3151363906 hasRelatedWork W104242109 @default.
- W3151363906 hasRelatedWork W1520363201 @default.
- W3151363906 hasRelatedWork W2006748418 @default.
- W3151363906 hasRelatedWork W2019166831 @default.
- W3151363906 hasRelatedWork W2348701290 @default.
- W3151363906 hasRelatedWork W2351344344 @default.
- W3151363906 hasRelatedWork W2355220228 @default.
- W3151363906 hasRelatedWork W2363660107 @default.
- W3151363906 hasRelatedWork W2365581031 @default.
- W3151363906 hasRelatedWork W2389460206 @default.
- W3151363906 hasRelatedWork W2389816032 @default.
- W3151363906 hasRelatedWork W2393564598 @default.
- W3151363906 hasRelatedWork W2397981011 @default.
- W3151363906 hasRelatedWork W2402214262 @default.
- W3151363906 hasRelatedWork W2419095988 @default.
- W3151363906 hasRelatedWork W2559594008 @default.
- W3151363906 hasRelatedWork W2981010969 @default.
- W3151363906 hasRelatedWork W2981143777 @default.
- W3151363906 hasRelatedWork W3141735523 @default.
- W3151363906 hasRelatedWork W2337208678 @default.
- W3151363906 isParatext "false" @default.
- W3151363906 isRetracted "false" @default.
- W3151363906 magId "3151363906" @default.
- W3151363906 workType "article" @default.