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- W3151366898 abstract "A high-throughput,simple and rapid chemiluminescence enzyme immunoassay(CLEIA)was developed for the clinical determination of progesterone in human serum,and the luminol-hydrogen peroxide was used as chemiluminescence system catalyzed by horseradish peroxidase(HRP).The solid phase of anti-progesterone antibody was prepared through the immunoreaction between anti-progesterone polyclonal antibody with donkey anti-rabbit IgG,i.e.second antibody,which had been physically absorbed on the wells of polystyrene microplate and was used as a universal solid phase.The effect of several factors,such as the dilution ratios of the immunoreagents,the chemiluminescent substrate,the chemiluminescence reaction time and incubation condition were examined and optimized.The optimal dilutions of anti-progesterone antibody and HRP-progesterone conjugate were 1∶10000 and 1∶15000,respectively.The substrate Ⅱ was chosen and the luminescence was determined after 10 min incubation.The immunoreaction was incubated in water bath at 37 ℃ for 1 h.The assay was evaluated with sensitivity as low as 0.08 μg/L.The RSD was less than 15% for both intra-and inter-assay precision.The recoveries of three different spiked concentration samples were 101%,101% and 94.4%,respectively.This proposed method has been successfully applied to the clinical evaluation of progesterone in 36 human sera.The results showed a good correlation with the commercially available radioimmunoassay kit with a correlative coefficient of 0.9502." @default.
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- W3151366898 date "2008-01-01" @default.
- W3151366898 modified "2023-09-25" @default.
- W3151366898 title "Micro-plate Chemiluminescence Enzyme Immunoassay for Clinical Determination of Progesterone in Human Serum" @default.
- W3151366898 hasPublicationYear "2008" @default.
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