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- W3151921222 abstract "Objective To explore the role of proteolytic stress induced by environmental toxins in degeneration and death of dopaminergic neurons.Methods Nerve growth factor-treated-rat adrenal pheochromocystoma cells of the line PC12 were co-incubated with 6-hydroxydopamine(6-OHDA),1- methyl-4 -phenylpyridinium ion(MPP~+),and rotenone for 24 hours.MTT assay was used to measure the cell viability induced by these neurotoxins with different concentrations.The expression levels of α-synuclein and ubiquited proteins in every group were observed with laser scanning confocal technique.The enzymatic activities of three main hydrolases in proteasome were measured by detection of the fluorophore of various cleft synthetic fluorogenic peptides.Results 6-OHDA,MPP~+,and rotenone decreased the activity of PC12 cells dose-dependently.Co-incubated with 100μmol/L 6-OHDA,75μmol/L MPP~+,and 20nmol/L rotenone,the activity of PC12 cells decreased by 52%,44%,and 40% respectively.Immunofluorescence double labeling confirmed the overexpression of α-synuclein and ubiquitin and pellet accumulation in the cytoplasm induced by three toxins.Compared with those in the control group,the trypsin-like,chymotrypsin- like,and PgH-like activities of proteasome were markedly decreased in the MPP~+ and rotenone groups(all P0.05)and slightly decreased in the 6-OHDA 100μmol/L group(all P0.05),but after the exposure to 6-OHDA 200μmol/L for 24h,the activities of the three enzymesl decreased rapidly,the activities of the three enzymes were 7.2±0.6,79.6±2.7 and 4.2±0.5 FU/100μg respectively(vs 13.9±1.8,99.3± 5.2,and 6.9±0.6 FU/100μg respectively in the control group,all P0.01).Conclusion Environmental toxins induce proteolytic stress marked by dysfunction of ubiquitin proteasome and accumulation of α-synuclein and ubiquited proteins." @default.
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- W3151921222 date "2007-01-01" @default.
- W3151921222 modified "2023-09-23" @default.
- W3151921222 title "Proteolytic stress induced by environmental toxins in dopaminergic neurons:an experimental study" @default.
- W3151921222 hasPublicationYear "2007" @default.
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