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- W3152012382 abstract "Theeffect ofmedia conditioned bymuscle cells onthedevelopment invitro ofchicken spinal neurons wasstudied. Neural tube cells of4.5-day chicken embryos weredissociated after trypsinization andcultured inserum-free minimum essential medium conditioned for4days over cultures offused chicken myotubes. After 20hrinconditioned medium (protein concentra- tion, 10-50 ftg/ml), about 50%ofsurviving cells hadextended neurites, whereas incultures innonconditioned medium this value wasabout 10%. Theactive factor(s) inconditioned medium ismac- romolecular andits activity wascompletely destroyed byincu- bation with trypsin. Concentrated samples ofconditioned medium wereanalyzed bygelfiltration oncolumns ofSepharose CL-6B. Theactivity wasrecovered inpeaks with apparent molecular weights of40,000 and500,000 andattheexclusion volume ofthe column. Mediaconditioned bychicken liver andskin cells also contained neurite-promoting activity butatlower levels. Noac- tivity wasdetected inNerve Growth Factor, insulin, fetal calf serum, orhorse serum orinmedia conditioned bychicken lung, chicken heart, orC6glioma cells." @default.
- W3152012382 created "2021-04-13" @default.
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- W3152012382 date "1981-01-01" @default.
- W3152012382 modified "2023-09-27" @default.
- W3152012382 title "Neurite outgrowth fromembryonic chicken spinal neurons is promoted bymediaconditioned bymuscle cells" @default.
- W3152012382 hasPublicationYear "1981" @default.
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