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- W3152061006 abstract "The aim of this study was to investigate the effect of evodiamine on the proliferation and the immune function of thymocytes and splenocyte of mice from three germlines,which were8weeks old masculinity BALB/c,C57BL/6and F1hybridization mice.Cells of thymus and spleen were harvested and prepared as unicellular suspension.Cell proliferation was detected by MTT method,while the concentration of IL-2was detected by ELISA,mRNA levels of bcl-2and cdk2in cells treated with evodiamine were detected by RT-PCR,the apoptosis rate and intracellular reactive oxygen species (ROS) concentration were amalyzed by FCM,and the protein levels of BCL-2,CDK2and BAX were determined by fluorescence microscope.The results indicated that at0.5,0.75 and 1μmol/L,evodiamine inhibited the proliferation and externalization of thymocytes and splenocytes stimulated with ConA (p0.05).At0.75μmol/L,evodiamine inhibited the secretion of IL-2,decreased the mRNA level of bcl-2 and cdk2,and induced apoptosis of thymocytes and splenocytes (p0.05).Intracellular ROS concentration increased significantly after treatment with evodiamine for12hours (p0.05).The death rate increased at a prolong period of time.After treatment with evodiamine for24and48hours,the cells were divided into two groups,one of which was negatively stained by2 7-dichlorofluorescein (DCF),which indicated that ROS level decreased significantly in the dying cells.It is concluded that evodiamine inhibits proliferation and induces apoptosis of thymocytes and splenocytes from different germline mice,and at the same time decreases secretion of IL-2 through down-regulating bcl-2 and cdk2levels." @default.
- W3152061006 created "2021-04-13" @default.
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- W3152061006 date "2008-01-01" @default.
- W3152061006 modified "2023-09-23" @default.
- W3152061006 title "Immunoregulatory Effect of Evodiamine in Mice of Various Germlines" @default.
- W3152061006 hasPublicationYear "2008" @default.
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