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- W3152114441 abstract "瞄准：为了与胎儿的肝细胞(FLC ) 和可能性在合作文化调查间充质的干细胞(MSC ) 的 hepatocytic 区别膨胀，区分了 hepatocytic 房间。方法：MSC 被制动火箭与绿荧光灯的蛋白质(GFP ) 标记病毒的基因转导变异。同种细胞的显著 MSC 在用与干细胞补充的fibronectin涂的培养皿和媒介刺激条件的肝下面是也有教养的因素( SCF )， hepatocyte 生长因素( HGF )，表皮的生长因素( EGF )，和成纤维细胞生长因素 4 ( FGF-4 )独自一个，或在刚孤立的 FLC 的存在。在合作文化的房间被收获，并且 GFP+ 或 GFP- 房间用荧光被分开激活的房间排序。为肝 specific 标记 cytokeratin-18 (CK-18 ) 的反向的抄写聚合酶链反应(RT-PCR )( 法新社) ，白朊，和 alpha-fetoprotein 在不同房间人口被执行。结果：在指定文化条件下面，与 FLC co 有教养的老鼠 MSC 超过二个星期表示了白朊， CK-18，和 AFP-RNA。在 wk 3， MSC 失去了 hepatocytic 基因表示，可能由于 cocultured FLC 的增生。FLC 也在合作文化和一个很高的生长潜力显示出稳定的肝 specific 基因表情。结论：从骨髓的老鼠 MSC 能面对 FLC 在试管内区分 hepatocytic 房间，在合作文化的 MSC 的存在也为 FLC 的扩大和区别提供有益的环境。" @default.
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- W3152114441 date "2006-01-01" @default.
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- W3152114441 title "Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells" @default.
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