Matches in SemOpenAlex for { <https://semopenalex.org/work/W3152117611> ?p ?o ?g. }
- W3152117611 abstract "Human pluripotent stem cells (PSCs), which are composed of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), provide an opportunity to advance cardiac cell therapy-based clinical trials. However, an important hurdle that must be overcome is the risk of teratoma formation after cell transplantation due to the proliferative capacity of residual undifferentiated PSCs in differentiation batches. To tackle this problem, we propose the use of a minimal noncardiotoxic doxorubicin dose as a purifying agent to selectively target rapidly proliferating stem cells for cell death, which will provide a purer population of terminally differentiated cardiomyocytes before cell transplantation. In this study, we determined an appropriate in vitro doxorubicin dose that (a) eliminates residual undifferentiated stem cells before cell injection to prevent teratoma formation after cell transplantation and (b) does not cause cardiotoxicity in ESC-derived cardiomyocytes (CMs) as demonstrated through contractility analysis, electrophysiology, topoisomerase activity assay, and quantification of reactive oxygen species generation. This study establishes a potentially novel method for tumorigenic-free cell therapy studies aimed at clinical applications of cardiac cell transplantation." @default.
- W3152117611 created "2021-04-13" @default.
- W3152117611 creator A5004876245 @default.
- W3152117611 creator A5012383046 @default.
- W3152117611 creator A5012538840 @default.
- W3152117611 creator A5014838924 @default.
- W3152117611 creator A5016320626 @default.
- W3152117611 creator A5019975484 @default.
- W3152117611 creator A5022629573 @default.
- W3152117611 creator A5030368165 @default.
- W3152117611 creator A5030635499 @default.
- W3152117611 creator A5047087199 @default.
- W3152117611 creator A5047561254 @default.
- W3152117611 creator A5060421375 @default.
- W3152117611 creator A5061644919 @default.
- W3152117611 creator A5064030220 @default.
- W3152117611 creator A5077069068 @default.
- W3152117611 date "2021-04-08" @default.
- W3152117611 modified "2023-10-18" @default.
- W3152117611 title "Method for selective ablation of undifferentiated human pluripotent stem cell populations for cell-based therapies" @default.
- W3152117611 cites W1899371747 @default.
- W3152117611 cites W1964179489 @default.
- W3152117611 cites W1994075121 @default.
- W3152117611 cites W1995737576 @default.
- W3152117611 cites W2020236947 @default.
- W3152117611 cites W2029753193 @default.
- W3152117611 cites W2041051519 @default.
- W3152117611 cites W2041991750 @default.
- W3152117611 cites W2050036109 @default.
- W3152117611 cites W2065653704 @default.
- W3152117611 cites W2066878307 @default.
- W3152117611 cites W2083599207 @default.
- W3152117611 cites W2094403994 @default.
- W3152117611 cites W2095859714 @default.
- W3152117611 cites W2105642488 @default.
- W3152117611 cites W2125987139 @default.
- W3152117611 cites W2142114378 @default.
- W3152117611 cites W2166305181 @default.
- W3152117611 cites W2170626923 @default.
- W3152117611 cites W2246145508 @default.
- W3152117611 cites W2268776220 @default.
- W3152117611 cites W2338021849 @default.
- W3152117611 cites W2346631874 @default.
- W3152117611 cites W2586749137 @default.
- W3152117611 cites W2596442933 @default.
- W3152117611 cites W2610026592 @default.
- W3152117611 cites W2685305227 @default.
- W3152117611 cites W2735324033 @default.
- W3152117611 cites W2886226762 @default.
- W3152117611 cites W2895606183 @default.
- W3152117611 cites W2899552791 @default.
- W3152117611 cites W2911982110 @default.
- W3152117611 cites W2952240309 @default.
- W3152117611 cites W2983246646 @default.
- W3152117611 cites W2999014832 @default.
- W3152117611 cites W3011173233 @default.
- W3152117611 cites W3011753122 @default.
- W3152117611 cites W3030059315 @default.
- W3152117611 doi "https://doi.org/10.1172/jci.insight.142000" @default.
- W3152117611 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8119193" @default.
- W3152117611 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33830086" @default.
- W3152117611 hasPublicationYear "2021" @default.
- W3152117611 type Work @default.
- W3152117611 sameAs 3152117611 @default.
- W3152117611 citedByCount "8" @default.
- W3152117611 countsByYear W31521176112021 @default.
- W3152117611 countsByYear W31521176112022 @default.
- W3152117611 countsByYear W31521176112023 @default.
- W3152117611 crossrefType "journal-article" @default.
- W3152117611 hasAuthorship W3152117611A5004876245 @default.
- W3152117611 hasAuthorship W3152117611A5012383046 @default.
- W3152117611 hasAuthorship W3152117611A5012538840 @default.
- W3152117611 hasAuthorship W3152117611A5014838924 @default.
- W3152117611 hasAuthorship W3152117611A5016320626 @default.
- W3152117611 hasAuthorship W3152117611A5019975484 @default.
- W3152117611 hasAuthorship W3152117611A5022629573 @default.
- W3152117611 hasAuthorship W3152117611A5030368165 @default.
- W3152117611 hasAuthorship W3152117611A5030635499 @default.
- W3152117611 hasAuthorship W3152117611A5047087199 @default.
- W3152117611 hasAuthorship W3152117611A5047561254 @default.
- W3152117611 hasAuthorship W3152117611A5060421375 @default.
- W3152117611 hasAuthorship W3152117611A5061644919 @default.
- W3152117611 hasAuthorship W3152117611A5064030220 @default.
- W3152117611 hasAuthorship W3152117611A5077069068 @default.
- W3152117611 hasBestOaLocation W31521176111 @default.
- W3152117611 hasConcept C104317684 @default.
- W3152117611 hasConcept C107459253 @default.
- W3152117611 hasConcept C10882517 @default.
- W3152117611 hasConcept C126322002 @default.
- W3152117611 hasConcept C143948831 @default.
- W3152117611 hasConcept C145103041 @default.
- W3152117611 hasConcept C1491633281 @default.
- W3152117611 hasConcept C2776694085 @default.
- W3152117611 hasConcept C2778233292 @default.
- W3152117611 hasConcept C2779389132 @default.
- W3152117611 hasConcept C28328180 @default.
- W3152117611 hasConcept C2908647359 @default.
- W3152117611 hasConcept C2911091166 @default.
- W3152117611 hasConcept C502942594 @default.
- W3152117611 hasConcept C55493867 @default.