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- W3152199711 abstract "Our previous research indicated that the Streptomyces pactum Act12(Act12) had a certain promotional effect on tanshinone accumulation and up-regulated the expression of genes 3-hydroxy-3-methyglutary1-CoA reductase(HMGR) and 1-deoxyd-xylulose-5-phosphate reductoisomerase(DXR) in Salvia miltiorrhiza hairy roots.This study focuses on the roles of reactive oxygen species in S.pactum Act12-induced tanshinone production in S.miltiorrhiza hairy roots.The 4% Act12,4% Act12 + CAT and 4%Act12 + SOD were added to S.miltiorrhiza hairy root and subcultured for 21 days,the dry weight,contents of reactive oxygen species,contents of tanshinones and expression of HMGR and DXR were determined at different harvest-time.The generation of reactive oxygen species(ROS) in S.miltiorrhiza hairy roots was triggered by 4% Act12 treatment.The relative expressions of genes HMGR and DXR in 4% Act12 treatment were 32.4 and 4.8-fold higher than those in the control.And the total tanshinone in the hairy roots was 10.2times higher than that of the control.The CAT and SOD could significantly inhibit the ROS accumulation and relative expressions of genes HMGR and DXR in 4% Act12 treatment,which induced the total tanshinone content was decreased by 74.6% comparing with the 4% Act12 treatment.ROS mediated Act12-induced tanshinone production.The Act12 may be via the ROS signal channel to activate the tanshinone biosynthesis pathways.Thereby the tanshinon content in hairy roots was increased." @default.
- W3152199711 created "2021-04-13" @default.
- W3152199711 creator A5062324262 @default.
- W3152199711 date "2014-01-01" @default.
- W3152199711 modified "2023-09-23" @default.
- W3152199711 title "Roles of reactive oxygen species in Streptomyces pactum Act12-induced tanshinone production in Salvia miltiorrhiza hairy roots" @default.
- W3152199711 hasPublicationYear "2014" @default.
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