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- W3152304476 abstract "This chapter summarizes the current advances in the field of protein chemical synthesis, which enable or facilitate the complete omission of high performance liquid chromatography purification as the major bottleneck in the progress of protein-based research. The strategies used include different variations of capture and release purification of peptide segments or ligated proteins, solid-phase chemical ligation of peptide segments to assemble large polypeptides/protein, and/or on-surface native chemical ligation strategies to rapidly assemble and interrogate immobilized protein libraries using a functional assay." @default.
- W3152304476 created "2021-04-13" @default.
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- W3152304476 date "2021-02-16" @default.
- W3152304476 modified "2023-09-27" @default.
- W3152304476 title "Toward HPLC ‐free Total Chemical Synthesis of Proteins" @default.
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- W3152304476 doi "https://doi.org/10.1002/9783527823567.ch8" @default.
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