FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3152754973> ?p ?o ?g. }

• W3152754973 abstract "The spread of antibiotic resistant Acinetobacter baumannii poses a significant threat to public health worldwide. This nosocomial bacterial pathogen can be associated with life-threatening infections, particularly in intensive care units. A. baumannii is mainly described as an extracellular pathogen with restricted survival within cells. This study shows that a subset of A. baumannii clinical isolates extensively multiply within non-phagocytic immortalized and primary cells, without the induction of apoptosis, and with bacterial clusters visible up to 48 hours after infection. This phenotype was observed for the A. baumannii C4 strain associated with high mortality in a hospital outbreak, and the A. baumannii ABC141 strain which wasn’t isolated from an infection site but was found to be hyperinvasive. Intracellular multiplication of these A. baumannii strains occurred within spacious single membrane-bound vacuoles, labeled with the lysosomal associate membrane protein (LAMP1). However, these compartments excluded lysotracker, an indicator of acidic pH, suggesting that A. baumannii can divert its trafficking away from the lysosomal degradative pathway. These compartments were also devoid of autophagy features. A high-content microscopy screen of 43 additional A. baumannii clinical strains highlighted various phenotypes: (1) the majority of strains remained extracellular, (2) a significant proportion was capable of invasion and limited persistence, and (3) two strains efficiently multiplied within LAMP1-positive vacuoles, one of which was also hyperinvasive. These data identify an intracellular niche for specific A. baumannii clinical strains that enables extensive multiplication in an environment protected from host immune responses and out of reach from many antibiotics. Importance Multidrug resistant Acinetobacter baumannii strains are associated with significant morbidity and mortality in hospitals world-wide. Understanding their pathogenicity is critical for improving therapeutics. Although A. baumannii can steadily adhere to surfaces and host cells, most bacteria remain extracellular. Recent studies have shown that a small proportion of bacteria can invade cells but present limited survival. We have found that some A. baumannii clinical isolates can establish a specialized intracellular niche that sustains extensive intracellular multiplication for a prolonged time without induction of cell death. We propose that this intracellular compartment allows A. baumannii to escape the cell’s normal degradative pathway, protecting bacteria from host immune responses and potentially hindering antibiotic accessibility. This may contribute to A. baumannii persistence, relapsing infections and enhanced mortality in susceptible patients. A high-content microscopy-based screen confirmed this pathogenicity trait is present in other clinical isolates. There is an urgent need for new antibiotics or alternative antimicrobial approaches, particularly to combat carbapenem-resistant A. baumannii . The discovery of an intracellular niche for this pathogen as well as hyperinvasive isolates may help guide the development of antimicrobial therapies and diagnostics in the future." @default.
• W3152754973 created "2021-04-26" @default.
• W3152754973 creator A5021645935 @default.
• W3152754973 creator A5025197915 @default.
• W3152754973 creator A5030342857 @default.
• W3152754973 creator A5042032186 @default.
• W3152754973 creator A5053083966 @default.
• W3152754973 creator A5058775083 @default.
• W3152754973 creator A5076324401 @default.
• W3152754973 creator A5077338095 @default.
• W3152754973 creator A5078578426 @default.
• W3152754973 creator A5078618500 @default.
• W3152754973 creator A5084244691 @default.
• W3152754973 date "2021-04-15" @default.
• W3152754973 modified "2023-10-18" @default.
• W3152754973 title "Incidence of an intracellular multiplication niche amongst <i>Acinetobacter baumannii</i> clinical isolates" @default.
• W3152754973 cites W1915856409 @default.
• W3152754973 cites W1964799586 @default.
• W3152754973 cites W1993933321 @default.
• W3152754973 cites W1998702177 @default.
• W3152754973 cites W2006850571 @default.
• W3152754973 cites W2024703363 @default.
• W3152754973 cites W2047860000 @default.
• W3152754973 cites W2084525915 @default.
• W3152754973 cites W2097302139 @default.
• W3152754973 cites W2098762865 @default.
• W3152754973 cites W2099540110 @default.
• W3152754973 cites W2102180009 @default.
• W3152754973 cites W2126480284 @default.
• W3152754973 cites W2140062393 @default.
• W3152754973 cites W2143195887 @default.
• W3152754973 cites W2146339643 @default.
• W3152754973 cites W2149018580 @default.
• W3152754973 cites W2149249163 @default.
• W3152754973 cites W2153766015 @default.
• W3152754973 cites W2158206104 @default.
• W3152754973 cites W2162573988 @default.
• W3152754973 cites W2167279371 @default.
• W3152754973 cites W2402574869 @default.
• W3152754973 cites W2491497270 @default.
• W3152754973 cites W2604659912 @default.
• W3152754973 cites W2621995756 @default.
• W3152754973 cites W2727121486 @default.
• W3152754973 cites W2795207345 @default.
• W3152754973 cites W2900040946 @default.
• W3152754973 cites W2907840621 @default.
• W3152754973 cites W2910616868 @default.
• W3152754973 cites W2962201066 @default.
• W3152754973 cites W2976296720 @default.
• W3152754973 cites W2989811897 @default.
• W3152754973 cites W3004741199 @default.
• W3152754973 cites W3038175478 @default.
• W3152754973 cites W3104572769 @default.
• W3152754973 cites W3150143214 @default.
• W3152754973 doi "https://doi.org/10.1101/2021.04.15.439986" @default.
• W3152754973 hasPublicationYear "2021" @default.
• W3152754973 type Work @default.
• W3152754973 sameAs 3152754973 @default.
• W3152754973 citedByCount "2" @default.
• W3152754973 countsByYear W31527549732023 @default.
• W3152754973 crossrefType "posted-content" @default.
• W3152754973 hasAuthorship W3152754973A5021645935 @default.
• W3152754973 hasAuthorship W3152754973A5025197915 @default.
• W3152754973 hasAuthorship W3152754973A5030342857 @default.
• W3152754973 hasAuthorship W3152754973A5042032186 @default.
• W3152754973 hasAuthorship W3152754973A5053083966 @default.
• W3152754973 hasAuthorship W3152754973A5058775083 @default.
• W3152754973 hasAuthorship W3152754973A5076324401 @default.
• W3152754973 hasAuthorship W3152754973A5077338095 @default.
• W3152754973 hasAuthorship W3152754973A5078578426 @default.
• W3152754973 hasAuthorship W3152754973A5078618500 @default.
• W3152754973 hasAuthorship W3152754973A5084244691 @default.
• W3152754973 hasBestOaLocation W31527549731 @default.
• W3152754973 hasConcept C102568950 @default.
• W3152754973 hasConcept C159047783 @default.
• W3152754973 hasConcept C190062978 @default.
• W3152754973 hasConcept C2776315533 @default.
• W3152754973 hasConcept C2776460866 @default.
• W3152754973 hasConcept C2777637488 @default.
• W3152754973 hasConcept C501593827 @default.
• W3152754973 hasConcept C523546767 @default.
• W3152754973 hasConcept C54355233 @default.
• W3152754973 hasConcept C79879829 @default.
• W3152754973 hasConcept C86803240 @default.
• W3152754973 hasConcept C89423630 @default.
• W3152754973 hasConceptScore W3152754973C102568950 @default.
• W3152754973 hasConceptScore W3152754973C159047783 @default.
• W3152754973 hasConceptScore W3152754973C190062978 @default.
• W3152754973 hasConceptScore W3152754973C2776315533 @default.
• W3152754973 hasConceptScore W3152754973C2776460866 @default.
• W3152754973 hasConceptScore W3152754973C2777637488 @default.
• W3152754973 hasConceptScore W3152754973C501593827 @default.
• W3152754973 hasConceptScore W3152754973C523546767 @default.
• W3152754973 hasConceptScore W3152754973C54355233 @default.
• W3152754973 hasConceptScore W3152754973C79879829 @default.
• W3152754973 hasConceptScore W3152754973C86803240 @default.
• W3152754973 hasConceptScore W3152754973C89423630 @default.
• W3152754973 hasLocation W31527549731 @default.
• W3152754973 hasLocation W31527549732 @default.
• W3152754973 hasOpenAccess W3152754973 @default.

• next