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- W3153157041 abstract "The cAMP-PKA signaling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to sudden nutrient transitions. We found that the characteristic cAMP peak differentiates between different carbon source transitions and is rather homogenous among single cells, especially for transitions to glucose. The peaks are mediated by a combination of extracellular sensing and intracellular metabolism. Moreover, the cAMP peak follows the Weber-Fechner law; its height scales with the relative, and not the absolute, change in glucose. Last, our results suggest that the cAMP peak height conveys information about prospective growth rates. In conclusion, our yEPAC-sensor makes possible new avenues for understanding yeast physiology, signaling, and metabolic adaptation." @default.
- W3153157041 created "2021-04-26" @default.
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- W3153157041 creator A5070958079 @default.
- W3153157041 creator A5085029333 @default.
- W3153157041 date "2021-06-15" @default.
- W3153157041 modified "2023-10-02" @default.
- W3153157041 title "A yeast FRET biosensor enlightens cAMP signaling" @default.
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- W3153157041 doi "https://doi.org/10.1091/mbc.e20-05-0319" @default.
- W3153157041 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8351543" @default.
- W3153157041 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33881352" @default.
- W3153157041 hasPublicationYear "2021" @default.
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