FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3153319005> ?p ?o ?g. }

• W3153319005 abstract "With the recent establishment of robust reverse genetics systems for rotavirus, rotavirus is being developed as a vector to express foreign genes. However, insertion of larger sequences such as those encoding multiple foreign genes into the rotavirus genome has been challenging because the virus segments are small. In this paper, we attempted to insert multiple foreign genes into a single gene segment of rotavirus to determine whether it can efficiently express multiple exogenous genes from its genome. At first, we engineered a truncated NSP1 segment platform lacking most of the NSP1 open reading frame and including a self-cleaving 2A sequence (2A), which made it possible to generate a recombinant rotavirus stably expressing NanoLuc (Nluc) luciferase as a model foreign gene. Based on this approach, we then demonstrated the generation of a replication-competent recombinant rotavirus expressing three reporter genes (Nluc, EGFP, and mCherry) by separating them with self-cleaving 2As, indicating the capacity of rotaviruses as to the insertion of multiple foreign genes. Importantly, the inserted multiple foreign genes remained genetically stable during serial passages in cell culture, indicating the potential of rotaviruses as attractive expression vectors. The strategy described here will serve as a model for the generation of rotavirus-based vectors designed for the expression and/or delivery of multiple foreign genes." @default.
• W3153319005 created "2021-04-26" @default.
• W3153319005 creator A5000206621 @default.
• W3153319005 creator A5000739053 @default.
• W3153319005 creator A5019780517 @default.
• W3153319005 creator A5033365237 @default.
• W3153319005 creator A5037719759 @default.
• W3153319005 creator A5040066413 @default.
• W3153319005 creator A5054167604 @default.
• W3153319005 creator A5069937286 @default.
• W3153319005 creator A5072190885 @default.
• W3153319005 date "2021-04-12" @default.
• W3153319005 modified "2023-10-15" @default.
• W3153319005 title "Strategy for generation of replication–competent recombinant rotaviruses expressing multiple foreign genes" @default.
• W3153319005 cites W1512932534 @default.
• W3153319005 cites W1614473236 @default.
• W3153319005 cites W1828960507 @default.
• W3153319005 cites W1930745477 @default.
• W3153319005 cites W1962555065 @default.
• W3153319005 cites W1980436917 @default.
• W3153319005 cites W1982048287 @default.
• W3153319005 cites W2000585490 @default.
• W3153319005 cites W2023545713 @default.
• W3153319005 cites W2025646139 @default.
• W3153319005 cites W2055075064 @default.
• W3153319005 cites W2058015620 @default.
• W3153319005 cites W2072328124 @default.
• W3153319005 cites W2075182411 @default.
• W3153319005 cites W2077354786 @default.
• W3153319005 cites W2112072874 @default.
• W3153319005 cites W2130052699 @default.
• W3153319005 cites W2145494049 @default.
• W3153319005 cites W2328134362 @default.
• W3153319005 cites W2474039476 @default.
• W3153319005 cites W2580855711 @default.
• W3153319005 cites W2615853094 @default.
• W3153319005 cites W2742793290 @default.
• W3153319005 cites W2799490329 @default.
• W3153319005 cites W2886376263 @default.
• W3153319005 cites W2912199523 @default.
• W3153319005 cites W2950249582 @default.
• W3153319005 cites W2972664546 @default.
• W3153319005 cites W2978534770 @default.
• W3153319005 cites W2990923188 @default.
• W3153319005 cites W3029970253 @default.
• W3153319005 cites W3034662783 @default.
• W3153319005 cites W3037234167 @default.
• W3153319005 cites W3038569761 @default.
• W3153319005 cites W3042534119 @default.
• W3153319005 doi "https://doi.org/10.1099/jgv.0.001587" @default.
• W3153319005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33843576" @default.
• W3153319005 hasPublicationYear "2021" @default.
• W3153319005 type Work @default.
• W3153319005 sameAs 3153319005 @default.
• W3153319005 citedByCount "4" @default.
• W3153319005 countsByYear W31533190052021 @default.
• W3153319005 countsByYear W31533190052022 @default.
• W3153319005 crossrefType "journal-article" @default.
• W3153319005 hasAuthorship W3153319005A5000206621 @default.
• W3153319005 hasAuthorship W3153319005A5000739053 @default.
• W3153319005 hasAuthorship W3153319005A5019780517 @default.
• W3153319005 hasAuthorship W3153319005A5033365237 @default.
• W3153319005 hasAuthorship W3153319005A5037719759 @default.
• W3153319005 hasAuthorship W3153319005A5040066413 @default.
• W3153319005 hasAuthorship W3153319005A5054167604 @default.
• W3153319005 hasAuthorship W3153319005A5069937286 @default.
• W3153319005 hasAuthorship W3153319005A5072190885 @default.
• W3153319005 hasConcept C104317684 @default.
• W3153319005 hasConcept C127413603 @default.
• W3153319005 hasConcept C141231307 @default.
• W3153319005 hasConcept C150194340 @default.
• W3153319005 hasConcept C159047783 @default.
• W3153319005 hasConcept C161733203 @default.
• W3153319005 hasConcept C167625842 @default.
• W3153319005 hasConcept C2522874641 @default.
• W3153319005 hasConcept C2780098074 @default.
• W3153319005 hasConcept C2781367151 @default.
• W3153319005 hasConcept C40767141 @default.
• W3153319005 hasConcept C47289529 @default.
• W3153319005 hasConcept C54355233 @default.
• W3153319005 hasConcept C70721500 @default.
• W3153319005 hasConcept C78519656 @default.
• W3153319005 hasConcept C86803240 @default.
• W3153319005 hasConceptScore W3153319005C104317684 @default.
• W3153319005 hasConceptScore W3153319005C127413603 @default.
• W3153319005 hasConceptScore W3153319005C141231307 @default.
• W3153319005 hasConceptScore W3153319005C150194340 @default.
• W3153319005 hasConceptScore W3153319005C159047783 @default.
• W3153319005 hasConceptScore W3153319005C161733203 @default.
• W3153319005 hasConceptScore W3153319005C167625842 @default.
• W3153319005 hasConceptScore W3153319005C2522874641 @default.
• W3153319005 hasConceptScore W3153319005C2780098074 @default.
• W3153319005 hasConceptScore W3153319005C2781367151 @default.
• W3153319005 hasConceptScore W3153319005C40767141 @default.
• W3153319005 hasConceptScore W3153319005C47289529 @default.
• W3153319005 hasConceptScore W3153319005C54355233 @default.
• W3153319005 hasConceptScore W3153319005C70721500 @default.
• W3153319005 hasConceptScore W3153319005C78519656 @default.
• W3153319005 hasConceptScore W3153319005C86803240 @default.
• W3153319005 hasFunder F4320309807 @default.

• next