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• W3153334459 abstract "Background. The molecular profiling of complex microbial communities has become the basis for examining the relationship between the microbiome composition, structure and metabolic functions of those communities. Microbial community structure can be partially assessed with “universal” PCR targeting taxonomic or functional gene markers. Increasingly, shotgun metagenomic DNA sequencing is providing more quantitative insight into microbiomes. However, both amplicon-based and shotgun sequencing approaches have shortcomings that limit the ability to study microbiome dynamics. Methods. We present a novel, amplicon-free, hybridization-based method (CaptureSeq) for profiling complex microbial communities using probes based on the chaperonin-60 gene. Molecular profiles of a commercially available synthetic microbial community standard were compared using CaptureSeq, whole metagenome sequencing, and 16S universal target amplification. Profiles were also generated for natural ecosystems including antibiotic-amended soils, manure storage tanks, and an agricultural reservoir. Results. The CaptureSeq method generated a microbial profile that encompassed all of the bacteria and eukaryotes in the panel with greater reproducibility and more accurate representation of high G/C content microorganisms compared to 16S amplification. In the natural ecosystems, CaptureSeq provided a much greater depth of coverage and sensitivity of detection compared to shotgun sequencing without prior selection. The resulting community profiles provided quantitatively reliable information about all three domains of life (Bacteria, Archaea, and Eukarya) in the different ecosystems. The applications of CaptureSeq will facilitate accurate studies of host-microbiome interactions for environmental, crop, animal and human health. Conclusions: cpn60-based hybridization enriched for taxonomically informative DNA sequences from complex mixtures. In synthetic and natural microbial ecosystems, CaptureSeq provided sequences from prokaryotes and eukaryotes simultaneously, with quantitatively reliable read abundances. CaptureSeq provides an alternative to PCR amplification of taxonomic markers with deep community coverage while minimizing amplification biases." @default.
• W3153334459 created "2021-04-26" @default.
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• W3153334459 date "2021-04-13" @default.
• W3153334459 modified "2023-10-02" @default.
• W3153334459 title "CaptureSeq: Hybridization-Based Enrichment of cpn60 Gene Fragments Reveals the Community Structures of Synthetic and Natural Microbial Ecosystems" @default.
• W3153334459 cites W1679625911 @default.
• W3153334459 cites W1846702953 @default.
• W3153334459 cites W1969346416 @default.
• W3153334459 cites W1970258769 @default.
• W3153334459 cites W1986358771 @default.
• W3153334459 cites W1989445815 @default.
• W3153334459 cites W1991936590 @default.
• W3153334459 cites W1997863428 @default.
• W3153334459 cites W1998928177 @default.
• W3153334459 cites W2005065536 @default.
• W3153334459 cites W2009170813 @default.
• W3153334459 cites W2009906332 @default.
• W3153334459 cites W2010210794 @default.
• W3153334459 cites W2010928292 @default.
• W3153334459 cites W2013635222 @default.
• W3153334459 cites W2019163239 @default.
• W3153334459 cites W2020281045 @default.
• W3153334459 cites W2021024989 @default.
• W3153334459 cites W2036897871 @default.
• W3153334459 cites W2039528162 @default.
• W3153334459 cites W2044633264 @default.
• W3153334459 cites W2053170703 @default.
• W3153334459 cites W2059241844 @default.
• W3153334459 cites W2070826422 @default.
• W3153334459 cites W2072970694 @default.
• W3153334459 cites W2075579489 @default.
• W3153334459 cites W2087544933 @default.
• W3153334459 cites W2095624327 @default.
• W3153334459 cites W2098386120 @default.
• W3153334459 cites W2099901798 @default.
• W3153334459 cites W2103923544 @default.
• W3153334459 cites W2108718991 @default.
• W3153334459 cites W2110375946 @default.
• W3153334459 cites W2110578246 @default.
• W3153334459 cites W2112874908 @default.
• W3153334459 cites W2112888168 @default.
• W3153334459 cites W2115741180 @default.
• W3153334459 cites W2121854298 @default.
• W3153334459 cites W2123152317 @default.
• W3153334459 cites W2123497476 @default.
• W3153334459 cites W2124985265 @default.
• W3153334459 cites W2127320820 @default.
• W3153334459 cites W2131186249 @default.
• W3153334459 cites W2137177315 @default.
• W3153334459 cites W2138527737 @default.
• W3153334459 cites W2153133935 @default.
• W3153334459 cites W2157107905 @default.
• W3153334459 cites W2166865790 @default.
• W3153334459 cites W2167292592 @default.
• W3153334459 cites W2176371198 @default.
• W3153334459 cites W2180202274 @default.
• W3153334459 cites W2207819727 @default.
• W3153334459 cites W2237523483 @default.
• W3153334459 cites W2257156795 @default.
• W3153334459 cites W2258031998 @default.
• W3153334459 cites W2319251666 @default.
• W3153334459 cites W2335786001 @default.
• W3153334459 cites W2401404581 @default.
• W3153334459 cites W2499317024 @default.
• W3153334459 cites W2516350521 @default.
• W3153334459 cites W2554002799 @default.
• W3153334459 cites W2559753870 @default.
• W3153334459 cites W2603930527 @default.
• W3153334459 cites W2733214817 @default.
• W3153334459 cites W2748607273 @default.
• W3153334459 cites W2762260969 @default.
• W3153334459 cites W2766085454 @default.
• W3153334459 cites W2794776675 @default.
• W3153334459 cites W2803457956 @default.
• W3153334459 cites W2808092359 @default.
• W3153334459 cites W2905443741 @default.
• W3153334459 cites W2920215701 @default.
• W3153334459 cites W2992668234 @default.
• W3153334459 cites W3041873878 @default.
• W3153334459 cites W3143312462 @default.
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• W3153334459 doi "https://doi.org/10.3390/microorganisms9040816" @default.
• W3153334459 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8069376" @default.
• W3153334459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33924343" @default.
• W3153334459 hasPublicationYear "2021" @default.
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