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• W3153861350 abstract "Background and AimsCrohn’s disease and ulcerative colitis are characterized by dysregulated adaptive immune responses to the microbiota in genetically susceptible individuals, but the specificity of these responses remains largely undefined. Therefore, we developed a microbiota antigen microarray to characterize microbial antibody reactivity, particularly to human-derived microbiota flagellins, in inflammatory bowel disease.MethodsSera from healthy volunteers (n = 87) at the University of Alabama at Birmingham and from patients recruited from the Kirklin Clinic of University of Alabama at Birmingham Hospital, including patients with Crohn’s disease (n = 152) and ulcerative colitis (n = 170), were individually probed against microbiota bacterial flagellins of both mouse and human origin and analyzed for IgG and IgA antibody responses. Circulating flagellin-reactive T effector (CD4+CD154+) and T regulatory (CD4+CD137+) cells were isolated and evaluated in selected patients. Resulting adaptive immune responses were compared with corresponding clinical data to determine relevancy to disease behavior.ResultsWe show that patients with IBD express selective patterns of antibody reactivity to microbiota flagellins. Patients with Crohn’s disease, but not patients with ulcerative colitis, display augmented serum IgG to human ileal-localized Lachnospiraceae flagellins, with a subset of patients having high responses to more than 10 flagellins. Elevated responses to CBir1, a mouse Lachnospiraceae flagellin used clinically to diagnose CD, correlated with multi-Lachnospiraceae flagellin reactivity. In this subset of patients with CD, multi-flagellin reactivity was associated with elevated flagellin-specific CD154+CD45RA– T memory cells, a reduced ratio of flagellin-reactive CD4+ T regulatory to T effector cells, and a high frequency of disease complications.ConclusionsPatients with Crohn’s disease display strong adaptive immune response to human-derived Lachnospiraceae flagellins, which may be targeted for prognosis and future personalized therapies. Crohn’s disease and ulcerative colitis are characterized by dysregulated adaptive immune responses to the microbiota in genetically susceptible individuals, but the specificity of these responses remains largely undefined. Therefore, we developed a microbiota antigen microarray to characterize microbial antibody reactivity, particularly to human-derived microbiota flagellins, in inflammatory bowel disease. Sera from healthy volunteers (n = 87) at the University of Alabama at Birmingham and from patients recruited from the Kirklin Clinic of University of Alabama at Birmingham Hospital, including patients with Crohn’s disease (n = 152) and ulcerative colitis (n = 170), were individually probed against microbiota bacterial flagellins of both mouse and human origin and analyzed for IgG and IgA antibody responses. Circulating flagellin-reactive T effector (CD4+CD154+) and T regulatory (CD4+CD137+) cells were isolated and evaluated in selected patients. Resulting adaptive immune responses were compared with corresponding clinical data to determine relevancy to disease behavior. We show that patients with IBD express selective patterns of antibody reactivity to microbiota flagellins. Patients with Crohn’s disease, but not patients with ulcerative colitis, display augmented serum IgG to human ileal-localized Lachnospiraceae flagellins, with a subset of patients having high responses to more than 10 flagellins. Elevated responses to CBir1, a mouse Lachnospiraceae flagellin used clinically to diagnose CD, correlated with multi-Lachnospiraceae flagellin reactivity. In this subset of patients with CD, multi-flagellin reactivity was associated with elevated flagellin-specific CD154+CD45RA– T memory cells, a reduced ratio of flagellin-reactive CD4+ T regulatory to T effector cells, and a high frequency of disease complications. Patients with Crohn’s disease display strong adaptive immune response to human-derived Lachnospiraceae flagellins, which may be targeted for prognosis and future personalized therapies." @default.
• W3153861350 created "2021-04-26" @default.
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• W3153861350 date "2021-08-01" @default.
• W3153861350 modified "2023-10-16" @default.
• W3153861350 title "Human Microbiota Flagellins Drive Adaptive Immune Responses in Crohn’s Disease" @default.
• W3153861350 cites W1576057902 @default.
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• W3153861350 cites W1972449088 @default.
• W3153861350 cites W1979217507 @default.
• W3153861350 cites W1981181890 @default.
• W3153861350 cites W1989502587 @default.
• W3153861350 cites W1989972789 @default.
• W3153861350 cites W1999283861 @default.
• W3153861350 cites W1999906527 @default.
• W3153861350 cites W2005699689 @default.
• W3153861350 cites W2006761088 @default.
• W3153861350 cites W2007631222 @default.
• W3153861350 cites W2007759930 @default.
• W3153861350 cites W2015272833 @default.
• W3153861350 cites W2026074634 @default.
• W3153861350 cites W2030364983 @default.
• W3153861350 cites W2031310375 @default.
• W3153861350 cites W2033960912 @default.
• W3153861350 cites W2034890198 @default.
• W3153861350 cites W2051312690 @default.
• W3153861350 cites W2057759034 @default.
• W3153861350 cites W2066203385 @default.
• W3153861350 cites W2068234766 @default.
• W3153861350 cites W2068734008 @default.
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• W3153861350 cites W2102013054 @default.
• W3153861350 cites W2102667210 @default.
• W3153861350 cites W2120522976 @default.
• W3153861350 cites W2122316223 @default.
• W3153861350 cites W2126131092 @default.
• W3153861350 cites W2127676534 @default.
• W3153861350 cites W2129831399 @default.
• W3153861350 cites W2134617060 @default.
• W3153861350 cites W2141125135 @default.
• W3153861350 cites W2141421205 @default.
• W3153861350 cites W2152217578 @default.
• W3153861350 cites W2153639094 @default.
• W3153861350 cites W2166691283 @default.
• W3153861350 cites W2167566687 @default.
• W3153861350 cites W2188248802 @default.
• W3153861350 cites W2199672799 @default.
• W3153861350 cites W2323895858 @default.
• W3153861350 cites W2343155348 @default.
• W3153861350 cites W2344724546 @default.
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• W3153861350 cites W2424417851 @default.
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• W3153861350 doi "https://doi.org/10.1053/j.gastro.2021.03.064" @default.
• W3153861350 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8489510" @default.
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