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- W3153881330 endingPage "890" @default.
- W3153881330 startingPage "879" @default.
- W3153881330 abstract "Structural characterization of macromolecular assemblies is often limited by the transient nature of the interactions. The development of specific chemical tools to covalently tether interacting proteins to each other has played a major role in various fundamental discoveries in recent years. To this end, protein engineering techniques such as mutagenesis, incorporation of unnatural amino acids, and methods using synthetic substrate/cosubstrate derivatives were employed. In this review, we give an overview of both commonly used and recently developed biochemical methodologies for covalent stabilization of macromolecular complexes enabling structural investigation via crystallography, nuclear magnetic resonance, and cryo-electron microscopy. We divided the strategies into nonenzymatic- and enzymatic-driven cross-linking and further categorized them in either naturally occurring or engineered covalent linkage. This review offers a compilation of recent advances in diverse scientific fields where the structural characterization of macromolecular complexes was achieved by the aid of intermolecular covalent linkage." @default.
- W3153881330 created "2021-04-26" @default.
- W3153881330 creator A5009761288 @default.
- W3153881330 creator A5044072921 @default.
- W3153881330 creator A5091424308 @default.
- W3153881330 date "2021-04-16" @default.
- W3153881330 modified "2023-09-27" @default.
- W3153881330 title "Current Advances in Covalent Stabilization of Macromolecular Complexes for Structural Biology" @default.
- W3153881330 cites W1965378730 @default.
- W3153881330 cites W1966159041 @default.
- W3153881330 cites W1973711348 @default.
- W3153881330 cites W1977831839 @default.
- W3153881330 cites W1992079344 @default.
- W3153881330 cites W1993646391 @default.
- W3153881330 cites W1998120501 @default.
- W3153881330 cites W1999336886 @default.
- W3153881330 cites W2009885482 @default.
- W3153881330 cites W2010734457 @default.
- W3153881330 cites W2011489155 @default.
- W3153881330 cites W2011747741 @default.
- W3153881330 cites W2014660585 @default.
- W3153881330 cites W2024249948 @default.
- W3153881330 cites W2029479572 @default.
- W3153881330 cites W2029794768 @default.
- W3153881330 cites W2030979689 @default.
- W3153881330 cites W2032078921 @default.
- W3153881330 cites W2032432541 @default.
- W3153881330 cites W2033317613 @default.
- W3153881330 cites W2036227881 @default.
- W3153881330 cites W2037607907 @default.
- W3153881330 cites W2039090083 @default.
- W3153881330 cites W2044702107 @default.
- W3153881330 cites W2045174330 @default.
- W3153881330 cites W2052320715 @default.
- W3153881330 cites W2053929225 @default.
- W3153881330 cites W2056979628 @default.
- W3153881330 cites W2058788882 @default.
- W3153881330 cites W2069515129 @default.
- W3153881330 cites W2074103590 @default.
- W3153881330 cites W2087101665 @default.
- W3153881330 cites W2089627181 @default.
- W3153881330 cites W2092551313 @default.
- W3153881330 cites W2094362595 @default.
- W3153881330 cites W2097755834 @default.
- W3153881330 cites W2099335767 @default.
- W3153881330 cites W2103203123 @default.
- W3153881330 cites W2103647536 @default.
- W3153881330 cites W2110443342 @default.
- W3153881330 cites W2133819225 @default.
- W3153881330 cites W2141035944 @default.
- W3153881330 cites W2142823843 @default.
- W3153881330 cites W2146176174 @default.
- W3153881330 cites W2147526198 @default.
- W3153881330 cites W2147542927 @default.
- W3153881330 cites W2170036411 @default.
- W3153881330 cites W2174055199 @default.
- W3153881330 cites W2245906427 @default.
- W3153881330 cites W2256699391 @default.
- W3153881330 cites W2329915050 @default.
- W3153881330 cites W2400942913 @default.
- W3153881330 cites W2478205319 @default.
- W3153881330 cites W2529250862 @default.
- W3153881330 cites W2530082208 @default.
- W3153881330 cites W2560463443 @default.
- W3153881330 cites W2585679309 @default.
- W3153881330 cites W2590813565 @default.
- W3153881330 cites W2613065881 @default.
- W3153881330 cites W2734790238 @default.
- W3153881330 cites W2756970589 @default.
- W3153881330 cites W2757317176 @default.
- W3153881330 cites W2765949138 @default.
- W3153881330 cites W2766375531 @default.
- W3153881330 cites W2767025970 @default.
- W3153881330 cites W2770135693 @default.
- W3153881330 cites W2780354290 @default.
- W3153881330 cites W2785726904 @default.
- W3153881330 cites W2795648902 @default.
- W3153881330 cites W2805711807 @default.
- W3153881330 cites W2884683306 @default.
- W3153881330 cites W2887841597 @default.
- W3153881330 cites W2888644902 @default.
- W3153881330 cites W2889305184 @default.
- W3153881330 cites W2903568403 @default.
- W3153881330 cites W2920896051 @default.
- W3153881330 cites W2945194478 @default.
- W3153881330 cites W2949140641 @default.
- W3153881330 cites W2949265479 @default.
- W3153881330 cites W2950609079 @default.
- W3153881330 cites W2962948444 @default.
- W3153881330 cites W2963870305 @default.
- W3153881330 cites W2967502564 @default.
- W3153881330 cites W2971032307 @default.
- W3153881330 cites W3006502081 @default.
- W3153881330 cites W3006529223 @default.
- W3153881330 cites W3010864714 @default.
- W3153881330 cites W3011035493 @default.
- W3153881330 cites W3021801309 @default.
- W3153881330 cites W3024188993 @default.