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- W3154282499 abstract "A new modification of the conventional multiple sample loading (MSL) mode – sequential sample loading (SSL) – is suggested to enhance further the performance of the counter-current chromatography (CCC) separation processes. The sequential sample loading technique is simple and easy to implement: the continuous sample solution supply to a CCC column is alternated (interrupted) with short periods of the pure mobile phase supply. Periodic (batch) and continuous SSL CCC separations can be designed and implemented. In continuous processes, the sample solution loading is carried out in the form of separate series, consisting of a number of sequential sample solution loads. In this work, the modeling of the conventional multiple sample loading and the sequential sample loading counter-current chromatography is used to compare the two operating modes considered. Equations for the calculation of band profiles, the recovery yield and the purity are given. Equations are also derived permitting the calculation of the optimum operating parameters of the separation processes. It is shown that the use of sequential sample loading makes it possible to produce fractions of purified compounds with a much higher concentration than in the original sample solution. The simulations of the conventional multiple sample loading and the sequential sample loading counter-current chromatography separations are presented in “Mathcad” software." @default.
- W3154282499 created "2021-04-26" @default.
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- W3154282499 date "2021-06-01" @default.
- W3154282499 modified "2023-09-23" @default.
- W3154282499 title "A simple and highly efficient counter-current chromatography method for the isolation of concentrated fractions of compounds based on the sequential sample loading technique: Comparative theoretical study of conventional multiple and intermittent sample loading counter-current chromatography separations" @default.
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- W3154282499 doi "https://doi.org/10.1016/j.chroma.2021.462163" @default.
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