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- W3154299957 abstract "Diagnosing cystic fibrosis (CF) when sweat chloride is not in the CF range and less than 2 disease-causing CFTR mutations are found requires physiological CFTR assays, which are not always feasible or available. We developed a new physiological CFTR assay based on the morphological differences between rectal organoids from subjects with and without CF. In organoids from 167 subjects with and 22 without CF, two parameters derived from a semi-automated image analysis protocol (rectal organoid morphology analysis, ROMA) fully discriminated CF subjects with two disease-causing mutations from non-CF subjects (p<0.001). ROMA, feasible at all ages, can be centralised to improve standardisation." @default.
- W3154299957 created "2021-04-26" @default.
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- W3154299957 date "2021-04-15" @default.
- W3154299957 modified "2023-10-03" @default.
- W3154299957 title "Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis" @default.
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- W3154299957 doi "https://doi.org/10.1136/thoraxjnl-2020-216368" @default.
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