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• W3154593472 abstract "Immuno-electron microscopy (Immuno-EM) is a powerful tool for identifying molecular targets with ultrastructural details in biological specimens. However, technical barriers, such as the loss of ultrastructural integrity, the decrease in antigenicity, or artifacts in the handling process, hinder the widespread use of the technique by biomedical researchers. We developed a method to overcome such challenges by combining light and electron microscopy with immunolabeling based on Tokuyasu's method. Using cryo-sectioned biological specimens, target proteins with excellent antigenicity were first immunolabeled for confocal analysis, and then the same tissue sections were further processed for electron microscopy, which provided a well-preserved ultrastructure comparable to that obtained using conventional electron microscopy. Moreover, this method does not require specifically designed correlative light and electron microscopy (CLEM) devices but rather employs conventional confocal and electron microscopes; therefore, it can be easily applied in many biomedical studies." @default.
• W3154593472 created "2021-04-26" @default.
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• W3154593472 date "2021-04-20" @default.
• W3154593472 modified "2023-09-27" @default.
• W3154593472 title "Correlative Light and Electron Microscopy Using Frozen Section Obtained Using Cryo-Ultramicrotomy" @default.
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• W3154593472 doi "https://doi.org/10.3390/ijms22084273" @default.
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