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- W3154624311 abstract "The essential Smc5/6 complex is required in response to replication stress and is best known for ensuring the fidelity of homologous recombination. Using single-molecule tracking in live fission yeast to investigate Smc5/6 chromatin association, we show that Smc5/6 is chromatin associated in unchallenged cells and this depends on the non-SMC protein Nse6. We define a minimum of two Nse6-dependent sub-pathways, one of which requires the BRCT-domain protein Brc1. Using defined mutants in genes encoding the core Smc5/6 complex subunits, we show that the Nse3 double-stranded DNA binding activity and the arginine fingers of the two Smc5/6 ATPase binding sites are critical for chromatin association. Interestingly, disrupting the single-stranded DNA (ssDNA) binding activity at the hinge region does not prevent chromatin association but leads to elevated levels of gross chromosomal rearrangements during replication restart. This is consistent with a downstream function for ssDNA binding in regulating homologous recombination." @default.
- W3154624311 created "2021-04-26" @default.
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- W3154624311 date "2021-04-16" @default.
- W3154624311 modified "2023-10-03" @default.
- W3154624311 title "Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo" @default.
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- W3154624311 doi "https://doi.org/10.7554/elife.68579" @default.
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