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• W3155178276 abstract "The replication of SARS-CoV-2 and other coronaviruses depends on transcription of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous transcription. Recent studies have aimed to characterize the expression of subgenomic SARS-CoV-2 transcripts in order to investigate their clinical significance. Here, we describe a novel panel of reverse transcription droplet digital PCR (RT-ddPCR) assays designed to specifically quantify multiple different subgenomic SARS-CoV-2 transcripts and distinguish them from transcripts that do not arise from discontinuous transcription at each locus. These assays can be applied to samples from SARS-CoV-2 infected patients to better understand the regulation of SARS-CoV-2 transcription and how different sgRNAs may contribute to viral pathogenesis and clinical disease severity." @default.
• W3155178276 created "2021-04-26" @default.
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• W3155178276 date "2022-05-01" @default.
• W3155178276 modified "2023-10-14" @default.
• W3155178276 title "Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts" @default.
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• W3155178276 doi "https://doi.org/10.1016/j.ymeth.2021.04.011" @default.
• W3155178276 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8105137" @default.
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