Matches in SemOpenAlex for { <https://semopenalex.org/work/W3155280225> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W3155280225 endingPage "511" @default.
- W3155280225 startingPage "502" @default.
- W3155280225 abstract "This study was aimed to investigate the Open Reading Frame-112 (ORF-112) gene, which encoded for a hypothetical 218 amino acids protein in Streptomyces bacteria. A complete ORF-112 gene was synthesized, with addition of a 6xHis-Tag at the N-terminal location. The synthesized DNA nucleotides were sub-cloned into bacterial expression plasmid pBAT4. The pBAT4-ORF-112 plasmid transformed in bacterial cells BL21(De3)pLysS, intended for protein over expression, induced by isopropyl β- d-1-thiogalactopyranoside (IPTG). The IMAC affinity chromatography technique was deployed for protein purifications. Highly-purified fractions of ORF-112 were achieved by using affinity Ni++-columns. The purified ORF-112 protein was tested for possible biological activity. The SDS-PAGE analysis exhibited a soluble 30 kDa size purified ORF-112 protein which showed a slight gel shifting from the predicted size. The virulent activity test on purified fractions of ORF-112 was measured using the Disk Diffusion Test and it disclosed a clear zone formed in response to fungi Candida albicans growth. The data implies that the ORF-112 protein has an acceptable protective effect against the fungus C. albicans as compared to positive control ketoconazole (KCZ) (P < 0.05) while the protein has a significantly lower protective effect against the fungus than Itraconazole (ICZ) (P > 0.05). The results clarify the hypothetical ORF-112 protein is a novel protein with protective response effect against fungi cells C. albicans on disk-diffusion.test." @default.
- W3155280225 created "2021-04-26" @default.
- W3155280225 creator A5008956431 @default.
- W3155280225 creator A5028681273 @default.
- W3155280225 creator A5063873324 @default.
- W3155280225 date "2021-04-20" @default.
- W3155280225 modified "2023-09-30" @default.
- W3155280225 title "BIOACTIVITY CHARACTERIZATION OF PURIFIED RECOMBINANT HYPOTHETICAL PROTEIN CODED BY OPEN READING FRAME-112 OF STREPTOMYCES." @default.
- W3155280225 doi "https://doi.org/10.36103/ijas.v52i2.1314" @default.
- W3155280225 hasPublicationYear "2021" @default.
- W3155280225 type Work @default.
- W3155280225 sameAs 3155280225 @default.
- W3155280225 citedByCount "1" @default.
- W3155280225 countsByYear W31552802252023 @default.
- W3155280225 crossrefType "journal-article" @default.
- W3155280225 hasAuthorship W3155280225A5008956431 @default.
- W3155280225 hasAuthorship W3155280225A5028681273 @default.
- W3155280225 hasAuthorship W3155280225A5063873324 @default.
- W3155280225 hasBestOaLocation W31552802251 @default.
- W3155280225 hasConcept C104317684 @default.
- W3155280225 hasConcept C145081991 @default.
- W3155280225 hasConcept C153911025 @default.
- W3155280225 hasConcept C167625842 @default.
- W3155280225 hasConcept C170344550 @default.
- W3155280225 hasConcept C185592680 @default.
- W3155280225 hasConcept C22744801 @default.
- W3155280225 hasConcept C2780917455 @default.
- W3155280225 hasConcept C40767141 @default.
- W3155280225 hasConcept C47289529 @default.
- W3155280225 hasConcept C55493867 @default.
- W3155280225 hasConcept C86803240 @default.
- W3155280225 hasConcept C89423630 @default.
- W3155280225 hasConceptScore W3155280225C104317684 @default.
- W3155280225 hasConceptScore W3155280225C145081991 @default.
- W3155280225 hasConceptScore W3155280225C153911025 @default.
- W3155280225 hasConceptScore W3155280225C167625842 @default.
- W3155280225 hasConceptScore W3155280225C170344550 @default.
- W3155280225 hasConceptScore W3155280225C185592680 @default.
- W3155280225 hasConceptScore W3155280225C22744801 @default.
- W3155280225 hasConceptScore W3155280225C2780917455 @default.
- W3155280225 hasConceptScore W3155280225C40767141 @default.
- W3155280225 hasConceptScore W3155280225C47289529 @default.
- W3155280225 hasConceptScore W3155280225C55493867 @default.
- W3155280225 hasConceptScore W3155280225C86803240 @default.
- W3155280225 hasConceptScore W3155280225C89423630 @default.
- W3155280225 hasIssue "2" @default.
- W3155280225 hasLocation W31552802251 @default.
- W3155280225 hasOpenAccess W3155280225 @default.
- W3155280225 hasPrimaryLocation W31552802251 @default.
- W3155280225 hasRelatedWork W2046120782 @default.
- W3155280225 hasRelatedWork W2356222853 @default.
- W3155280225 hasRelatedWork W2359665887 @default.
- W3155280225 hasRelatedWork W2364705426 @default.
- W3155280225 hasRelatedWork W2373646174 @default.
- W3155280225 hasRelatedWork W2386002300 @default.
- W3155280225 hasRelatedWork W2386558435 @default.
- W3155280225 hasRelatedWork W2387272355 @default.
- W3155280225 hasRelatedWork W2389269354 @default.
- W3155280225 hasRelatedWork W3028847987 @default.
- W3155280225 hasVolume "52" @default.
- W3155280225 isParatext "false" @default.
- W3155280225 isRetracted "false" @default.
- W3155280225 magId "3155280225" @default.
- W3155280225 workType "article" @default.