FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3155653639> ?p ?o ?g. }

• W3155653639 abstract "Abstract Background Membrane protrusions that occur on the dorsal surface of a cell are an excellent experimental system to study actin machinery at work in a living cell. Small GTPase Rac1 controls the membrane protrusions that form and encapsulate extracellular volumes to perform pinocytic or phagocytic functions. Results Here, capitalizing on rapid volumetric imaging capabilities of lattice light-sheet microscopy (LLSM), we describe optogenetic approaches using photoactivable Rac1 (PA-Rac1) for controlled ruffle generation. We demonstrate that PA-Rac1 activation needs to be continuous, suggesting a threshold local concentration for sustained actin polymerization leading to ruffling. We show that Rac1 activation leads to actin assembly at the dorsal surface of the cell membrane that result in sheet-like protrusion formation without any requirement of a template. Further, this approach can be used to study the complex morpho-dynamics of the protrusions or to investigate specific proteins that may be enriched in the ruffles. Deactivating PA-Rac1 leads to complex contractile processes resulting in formation of macropinosomes. Using multicolour imaging in combination with these approaches, we find that Myo1e specifically is enriched in the ruffles. Conclusions Combining LLSM and optogenetics enables superior spatial and temporal control for studying such dynamic mechanisms. Demonstrated here, the techniques implemented provide insight into the complex nature of the molecular interplay involved in dynamic actin machinery, revealing that Rac1 activation can generate untemplated, lamellar protrusions." @default.
• W3155653639 created "2021-04-26" @default.
• W3155653639 creator A5014049129 @default.
• W3155653639 creator A5018550291 @default.
• W3155653639 creator A5030056688 @default.
• W3155653639 creator A5031153738 @default.
• W3155653639 creator A5039306388 @default.
• W3155653639 creator A5073742280 @default.
• W3155653639 creator A5082107271 @default.
• W3155653639 creator A5083915544 @default.
• W3155653639 creator A5090258951 @default.
• W3155653639 date "2021-04-13" @default.
• W3155653639 modified "2023-09-27" @default.
• W3155653639 title "Rac1 activation can generate untemplated, lamellar membrane ruffles" @default.
• W3155653639 cites W103612330 @default.
• W3155653639 cites W1561407320 @default.
• W3155653639 cites W1564175926 @default.
• W3155653639 cites W1571919210 @default.
• W3155653639 cites W1577015300 @default.
• W3155653639 cites W1915414973 @default.
• W3155653639 cites W1964350146 @default.
• W3155653639 cites W1967248496 @default.
• W3155653639 cites W1980971505 @default.
• W3155653639 cites W1981890485 @default.
• W3155653639 cites W1984575247 @default.
• W3155653639 cites W1991549216 @default.
• W3155653639 cites W1991738124 @default.
• W3155653639 cites W1995513355 @default.
• W3155653639 cites W2002939552 @default.
• W3155653639 cites W2010409953 @default.
• W3155653639 cites W2015615942 @default.
• W3155653639 cites W2026260372 @default.
• W3155653639 cites W2027146263 @default.
• W3155653639 cites W2029022457 @default.
• W3155653639 cites W2039205173 @default.
• W3155653639 cites W2040010587 @default.
• W3155653639 cites W2050140768 @default.
• W3155653639 cites W2073305879 @default.
• W3155653639 cites W2076174223 @default.
• W3155653639 cites W2079079774 @default.
• W3155653639 cites W2080405070 @default.
• W3155653639 cites W2080647249 @default.
• W3155653639 cites W2081758202 @default.
• W3155653639 cites W2087591542 @default.
• W3155653639 cites W2088909704 @default.
• W3155653639 cites W2089472706 @default.
• W3155653639 cites W2091792425 @default.
• W3155653639 cites W2093226717 @default.
• W3155653639 cites W2093844155 @default.
• W3155653639 cites W2095751049 @default.
• W3155653639 cites W2098011724 @default.
• W3155653639 cites W2105512502 @default.
• W3155653639 cites W2118785411 @default.
• W3155653639 cites W2123778917 @default.
• W3155653639 cites W2127488207 @default.
• W3155653639 cites W2136230579 @default.
• W3155653639 cites W2136776121 @default.
• W3155653639 cites W2167158605 @default.
• W3155653639 cites W2170608748 @default.
• W3155653639 cites W2621653801 @default.
• W3155653639 cites W2743101013 @default.
• W3155653639 cites W2759448220 @default.
• W3155653639 cites W2759963226 @default.
• W3155653639 cites W2802943473 @default.
• W3155653639 cites W2888906615 @default.
• W3155653639 cites W2923898779 @default.
• W3155653639 cites W2943127305 @default.
• W3155653639 cites W2969150624 @default.
• W3155653639 cites W3001778727 @default.
• W3155653639 cites W3081192047 @default.
• W3155653639 cites W4238726741 @default.
• W3155653639 cites W45421301 @default.
• W3155653639 doi "https://doi.org/10.1186/s12915-021-00997-3" @default.
• W3155653639 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8042924" @default.
• W3155653639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33849538" @default.
• W3155653639 hasPublicationYear "2021" @default.
• W3155653639 type Work @default.
• W3155653639 sameAs 3155653639 @default.
• W3155653639 citedByCount "11" @default.
• W3155653639 countsByYear W31556536392021 @default.
• W3155653639 countsByYear W31556536392022 @default.
• W3155653639 countsByYear W31556536392023 @default.
• W3155653639 crossrefType "journal-article" @default.
• W3155653639 hasAuthorship W3155653639A5014049129 @default.
• W3155653639 hasAuthorship W3155653639A5018550291 @default.
• W3155653639 hasAuthorship W3155653639A5030056688 @default.
• W3155653639 hasAuthorship W3155653639A5031153738 @default.
• W3155653639 hasAuthorship W3155653639A5039306388 @default.
• W3155653639 hasAuthorship W3155653639A5073742280 @default.
• W3155653639 hasAuthorship W3155653639A5082107271 @default.
• W3155653639 hasAuthorship W3155653639A5083915544 @default.
• W3155653639 hasAuthorship W3155653639A5090258951 @default.
• W3155653639 hasBestOaLocation W31556536391 @default.
• W3155653639 hasConcept C12554922 @default.
• W3155653639 hasConcept C125705527 @default.
• W3155653639 hasConcept C142669718 @default.
• W3155653639 hasConcept C1491633281 @default.
• W3155653639 hasConcept C169760540 @default.
• W3155653639 hasConcept C199528912 @default.
• W3155653639 hasConcept C2778106830 @default.

• next